Document Detail

Refractory Periodontitis Population Characterized by a Hyperactive Oral Neutrophil Phenotype.
MedLine Citation:
PMID:  21080789     Owner:  NLM     Status:  Publisher    
Background: Neutrophils, in addition to being the primary protective component of the innate immune system, also contribute to periodontal destruction through production of Reactive Oxygen Species (ROS) which cause damage to connective tissues and extra-cellular matrix following neutrophil activation. We have previously shown that hyperactive neutrophils are present in peripheral blood samples of patients diagnosed with refractory periodontitis. In order to test the hypothesis that oral neutrophil hyperactivity is related to periodontal disease severity, we utilized a flow cytometric approach to isolate and analyze oral neutrophil ROS (oROS) production in a refractory periodontal disease patient population. Methods: Oral rinse samples and venous blood were obtained from 13 patients diagnosed with refractory periodontitis. Following isolation of neutrophils from both samples, dihydrorhodamine 123 was used as a fluorescent probe for Phorbol 12-Myristate 13-Acetate mediated ROS production as assessed through flow cytometry. For each patient, oROS production levels were expressed as a percentage of their baseline to maximal peripheral blood neutrophil ROS production range. Results: Two distinct groups of refractory patients were identified based on levels of PMA stimulated oROS production. The patient group with high oROS production had significantly more clinical attachment loss compared to the patient group with low oROS production. Conclusions: Our findings demonstrate that a group of refractory patients with increased clinical attachment loss present with a hyperactive oral neutrophil phenotype characterized by increased potential for ROS production. Identification of this exaggerated oral neutrophil phenotype could allow clinicians to identify which patients are more susceptible to rapid disease progression.
Guy M Aboodi; Michael B Goldberg; Michael Glogauer
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-11-16
Journal Detail:
Title:  Journal of periodontology     Volume:  -     ISSN:  1943-3670     ISO Abbreviation:  J. Periodontol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Periodontology, Faculty of Dentistry, University of Toronto.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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