Document Detail

Refractile mycobacteria in Romanowsky-stained bone marrow smears. A comparison of acid-fast-stained tissue sections and Romanowsky-stained smears.
MedLine Citation:
PMID:  1371899     Owner:  NLM     Status:  MEDLINE    
The appearance of mycobacteria was studied in Wright-stained bone marrow preparations of human immunodeficiency virus-infected patients and compared with acid-fast-stained trephine biopsy sections and culture results. Mycobacterium avium complex in Romanowsky-stained preparations may be seen as extracellular and intracellular clear or red refractile beaded rods and nonrefractile "negative images." Refractile mycobacteria were seen in 17 of 20 culture-positive cases. Acid-fast stain of the trephine biopsy demonstrated organisms in only 11 of the 20 cases. Thus, six cases were culture positive and contained refractile rods but had no acid-fast organisms on the trephine biopsy. No false-positive results were seen with Romanowsky stain; the three false-negative results for refractility also were negative with acid-fast stain. Examination of Romanowsky-stained smears or imprints for refractile mycobacteria provides a reliable and sensitive method to identify mycobacteria in this population. Romanowsky-stained bone marrow aspirate and imprint smears should be examined for refractile bacilli when mycobacterial infection is suspected.
E Torlakovic; F Clayton; E D Ames
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  97     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-04-08     Completed Date:  1992-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  318-21     Citation Subset:  AIM; IM; X    
Department of Pathology, St. Luke's/Roosevelt Hospital, New York, New York 10019.
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MeSH Terms
Acquired Immunodeficiency Syndrome / microbiology
Azure Stains*
Bone Marrow / microbiology*,  pathology
Coloring Agents
Eosine Yellowish-(YS)*
Mycobacterium / isolation & purification*
Staining and Labeling*
Reg. No./Substance:
0/Azure Stains; 0/Coloring Agents; 0/Ferrocyanides; 0/Romanowsky-Giemsa stain; 14038-43-8/ferric ferrocyanide; 17372-87-1/Eosine Yellowish-(YS)

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