| Refractile mycobacteria in Romanowsky-stained bone marrow smears. A comparison of acid-fast-stained tissue sections and Romanowsky-stained smears. | |
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MedLine Citation:
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PMID: 1371899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The appearance of mycobacteria was studied in Wright-stained bone marrow preparations of human immunodeficiency virus-infected patients and compared with acid-fast-stained trephine biopsy sections and culture results. Mycobacterium avium complex in Romanowsky-stained preparations may be seen as extracellular and intracellular clear or red refractile beaded rods and nonrefractile "negative images." Refractile mycobacteria were seen in 17 of 20 culture-positive cases. Acid-fast stain of the trephine biopsy demonstrated organisms in only 11 of the 20 cases. Thus, six cases were culture positive and contained refractile rods but had no acid-fast organisms on the trephine biopsy. No false-positive results were seen with Romanowsky stain; the three false-negative results for refractility also were negative with acid-fast stain. Examination of Romanowsky-stained smears or imprints for refractile mycobacteria provides a reliable and sensitive method to identify mycobacteria in this population. Romanowsky-stained bone marrow aspirate and imprint smears should be examined for refractile bacilli when mycobacterial infection is suspected. |
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Authors:
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E Torlakovic; F Clayton; E D Ames |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 97 ISSN: 0002-9173 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 1992 Mar |
Date Detail:
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Created Date: 1992-04-08 Completed Date: 1992-04-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 318-21 Citation Subset: AIM; IM; X |
Affiliation:
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Department of Pathology, St. Luke's/Roosevelt Hospital, New York, New York 10019. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acquired Immunodeficiency Syndrome
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microbiology Azure Stains* Biopsy Bone Marrow / microbiology*, pathology Coloring Agents Eosine Yellowish-(YS)* Ferrocyanides Humans Mycobacterium / isolation & purification* Staining and Labeling* |
| Chemical | |
Reg. No./Substance:
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0/Azure Stains; 0/Coloring Agents; 0/Ferrocyanides; 0/Romanowsky-Giemsa stain; 14038-43-8/ferric ferrocyanide; 17372-87-1/Eosine Yellowish-(YS) |
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