Document Detail


Refolding of urea-denatured α-chymotrypsin by protein-folding liquid chromatography.
MedLine Citation:
PMID:  23033217     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An approach for re-folding denatured proteins during proteome research by protein folding liquid chromatography (PFLC) is presented. Standard protein, α-chymotrypsin (α-Chy), was selected as a model protein and hydrophobic interaction chromatography was performed as a typical PFLC; the three different α-Chy states - urea-denatured (U state), its folded intermediates (M state) and nature state (N state) - were studied during protein folding. Based on the test by matrix-assisted laser desorption/ionization time of flight mass spectrometry and bioactivity, only one stable M state of the α-Chy was identified and then it was prepared for further investigation. The specific bioactivity of the refolded α-Chy was found to be higher than that of commercial α-Chy as the urea concentration in the sample solution ranged from 1.0 to 3.0 m; the highest specific bioactivity at urea concentration was 1.0 m, indicating the possibility for re-folding some proteins that have partially or completely lost their bioactivity, as a dilute urea solution was employed for dissolving the sample. The experiment showed that the peak height of its M state increased with increasing urea concentration, and correspondingly decreased in the amount of the refolded α-Chy. When the urea concentration reached 6.0 m, the unfolded α-Chy could not be refolded at all. Copyright © 2012 John Wiley & Sons, Ltd.
Authors:
Ke Congyu; Sun Wujuan; Zhang Qunzheng; Geng Xindu
Related Documents :
23464857 - Self-assembled porphyrin nanodiscs with structure-dependent activation for phototherapy...
24797807 - An in vivo crosslinking approach to isolate protein complexes from drosophila embryos.
24204717 - Development-specific differences in the proteomics of angiostrongylus cantonensis.
4611927 - Protein synthesis by treponema pallidum extracted from infected rabbit tissue.
19704937 - Sp-d-dependent regulation of no metabolism in lipopolysaccharide-stimulated peritoneal ...
1772837 - Implementing knot-theoretical characterization methods to analyze the backbone structur...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-3
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  -     ISSN:  1099-0801     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Affiliation:
College of Chemistry & Chemical Engineering, Xi' An ShiYou University, Xi'an, 710065, China. kcy@xsyu.edu.cn.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Advanced Geometries of PEDOT Formed in Titania Nanotubes.
Next Document:  Synthesis of Amino, Azido, Nitro, and Nitrogen-Rich Azole Substituted Derivatives of 1H-Benzotriazol...