Document Detail


Reflectance-based determination of optical properties in highly attenuating tissue.
MedLine Citation:
PMID:  12683846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accurate data on in vivo tissue optical properties in the ultraviolet A (UVA) to visible (VIS) range are needed to elucidate light propagation effects and to aid in identifying safe exposure limits for biomedical optical spectroscopy. We have performed a preliminary study toward the development of a diffuse reflectance system with maximum fiber separation distance of less than 2.5 mm. The ultimate objective is to perform endoscopic measurement of optical properties in the UVA to VIS. Optical property sets with uniformly and randomly distributed values were developed within the range of interest: absorption coefficients from 1 to 25 cm(-1) and reduced scattering coefficients from 5 to 25 cm(-1). Reflectance datasets were generated by direct measurement of Intralipid-dye tissue phantoms at lambda=675 nm and Monte Carlo simulation of light propagation. Multivariate calibration models were generated using feed-forward artificial neural network or partial least squares algorithms. Models were calibrated and evaluated using simulated or measured reflectance datasets. The most accurate models developed-those based on a neural network and uniform optical property intervals-were able to determine absorption and reduced scattering coefficients with root mean square errors of +/-2 and +/-3 cm(-1), respectively. Measurements of ex vivo bovine liver at 543 and 633 nm were within 5 to 30% of values reported in the literature. While our technique for determination of optical properties appears feasible and moderately accurate, enhanced accuracy may be achieved through modification of the experimental system and processing algorithms.
Authors:
T Joshua Pfefer; L Stephanie Matchette; Carrie L Bennett; Jessica A Gall; Joy N Wilke; Anthony J Durkin; Marwood N Ediger
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Publication Detail:
Type:  Evaluation Studies; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Validation Studies    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  8     ISSN:  1083-3668     ISO Abbreviation:  J Biomed Opt     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-09     Completed Date:  2003-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  206-15     Citation Subset:  IM    
Affiliation:
US Food and Drug Administration, Center for Devices and Radiological Health, Rockville, Maryland 20857, USA. tjp@cdrh.fda.gov
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MeSH Terms
Descriptor/Qualifier:
Algorithms*
Animals
Calibration / standards
Cattle
Endoscopy / methods*,  standards
Image Enhancement / methods,  standards
Image Interpretation, Computer-Assisted / methods*,  standards
Light
Liver / physiology*
Models, Biological*
Neural Networks (Computer)*
Phantoms, Imaging
Scattering, Radiation
Spectrum Analysis / methods
Tomography, Optical / instrumentation,  methods*,  standards

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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