| Refinement of the pressure assay for milk urea nitrogen. | |
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MedLine Citation:
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PMID: 11003237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report improvements in the application of a pressure-based assay for urea. The assay involved the enzymatic hydrolysis of urea and subsequent measurement of CO2 partial pressure. Effects of the preservative bronopol on the assay and their implications for laboratory applications are discussed. A method of remediating these effects with cysteine is described. A method is also described wherein these additives can be used to prepare standards of known urea concentration in milk. The improved assay can be used to measure urea N in unadulterated milk or in bronopol preserved milk with an accuracy of +/-0.7 mg/dl (0.25 mM) in the range from 0 to 30 mg/dl (0 to 10.7 mM). |
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Authors:
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D M Jenkins; M J Delwiche; E J DePeters; R H BonDurant |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of dairy science Volume: 83 ISSN: 0022-0302 ISO Abbreviation: J. Dairy Sci. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2001-01-03 Completed Date: 2001-09-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2042-8 Citation Subset: IM |
Affiliation:
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Department of Biological and Agricultural Engineering, University of California at Davis, 95616, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoanalysis / veterinary Biosensing Techniques Carbon Dioxide / analysis* Cysteine / administration & dosage Glutamate Dehydrogenase / metabolism Linear Models Milk / chemistry* Nitrogen / analysis*, standards Partial Pressure Preservatives, Pharmaceutical / adverse effects Propylene Glycols / adverse effects Urea / analysis*, standards Urease / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Preservatives, Pharmaceutical; 0/Propylene Glycols; 124-38-9/Carbon Dioxide; 52-51-7/bronopol; 52-90-4/Cysteine; 57-13-6/Urea; 7727-37-9/Nitrogen; EC 1.4.1.2/Glutamate Dehydrogenase; EC 3.5.1.5/Urease |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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