Document Detail


Reference methods--with special reference to cholesterol.
MedLine Citation:
PMID:  2772557     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Through the work of the committee for reference methods in NORDKEM and the Scandinavian Society for Clinical Chemistry, a hierarchy of methods was established in Denmark in 1982 for accuracy assurance of determination of total cholesterol in serum. The hierarchy makes it possible for any laboratory to relate their results of measurements on fresh samples from humans, to the results obtained on the same samples by reference methods, which have been adjusted to definitive methodology. Proficiency studies in 1985 and 1988 gave consensus values (mean of all participants data) for total cholesterol in frozen human serum almost identical to values obtained by reference methods. Interlaboratory coefficients of variations were 4-5%. It is essential for the clinical chemist as well as for the clinician to be aware of the relative quantitative importance of preanalytical, analytical and biological variation and to realize to what extent replicate measurements on the same sample and on several samples from the same person reduce the total intraperson variation of serum cholesterol concentrations.
Authors:
S Stender
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation. Supplementum     Volume:  194     ISSN:  0085-591X     ISO Abbreviation:  Scand. J. Clin. Lab. Invest. Suppl.     Publication Date:  1989  
Date Detail:
Created Date:  1989-09-26     Completed Date:  1989-09-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  2984789R     Medline TA:  Scand J Clin Lab Invest Suppl     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  63-7     Citation Subset:  IM    
Affiliation:
Department of Clinical Chemistry, Hvidovre Hospital, University of Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Blood Chemical Analysis / standards*
Cholesterol / blood*
Humans
Chemical
Reg. No./Substance:
57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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