Document Detail


Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
MedLine Citation:
PMID:  21295703     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The developmental potential of human pluripotent stem cells suggests that they can produce disease-relevant cell types for biomedical research. However, substantial variation has been reported among pluripotent cell lines, which could affect their utility and clinical safety. Such cell-line-specific differences must be better understood before one can confidently use embryonic stem (ES) or induced pluripotent stem (iPS) cells in translational research. Toward this goal we have established genome-wide reference maps of DNA methylation and gene expression for 20 previously derived human ES lines and 12 human iPS cell lines, and we have measured the in vitro differentiation propensity of these cell lines. This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines. The combination of assays yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.
Authors:
Christoph Bock; Evangelos Kiskinis; Griet Verstappen; Hongcang Gu; Gabriella Boulting; Zachary D Smith; Michael Ziller; Gist F Croft; Mackenzie W Amoroso; Derek H Oakley; Andreas Gnirke; Kevin Eggan; Alexander Meissner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  144     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-07     Completed Date:  2011-03-10     Revised Date:  2013-09-19    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  439-52     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Broad Institute, Cambridge, MA 02142, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation
Cell Line
DNA Methylation*
Embryonic Stem Cells / cytology,  physiology*
Gene Expression Profiling / standards*
Humans
Induced Pluripotent Stem Cells / cytology,  physiology*
Grant Support
ID/Acronym/Agency:
U01 ES017155/ES/NIEHS NIH HHS; U01 ES017155-04/ES/NIEHS NIH HHS; U01ES017155/ES/NIEHS NIH HHS
Comments/Corrections
Comment In:
Nat Rev Genet. 2011 Mar;12(3):153   [PMID:  21331086 ]
Nat Methods. 2011 Apr;8(4):287   [PMID:  21574272 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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