| Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines. | |
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MedLine Citation:
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PMID: 21295703 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The developmental potential of human pluripotent stem cells suggests that they can produce disease-relevant cell types for biomedical research. However, substantial variation has been reported among pluripotent cell lines, which could affect their utility and clinical safety. Such cell-line-specific differences must be better understood before one can confidently use embryonic stem (ES) or induced pluripotent stem (iPS) cells in translational research. Toward this goal we have established genome-wide reference maps of DNA methylation and gene expression for 20 previously derived human ES lines and 12 human iPS cell lines, and we have measured the in vitro differentiation propensity of these cell lines. This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines. The combination of assays yields a scorecard for quick and comprehensive characterization of pluripotent cell lines. |
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Authors:
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Christoph Bock; Evangelos Kiskinis; Griet Verstappen; Hongcang Gu; Gabriella Boulting; Zachary D Smith; Michael Ziller; Gist F Croft; Mackenzie W Amoroso; Derek H Oakley; Andreas Gnirke; Kevin Eggan; Alexander Meissner |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell Volume: 144 ISSN: 1097-4172 ISO Abbreviation: Cell Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-07 Completed Date: 2011-03-10 Revised Date: 2012-02-09 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
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Languages: eng Pagination: 439-52 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Broad Institute, Cambridge, MA 02142, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Differentiation Cell Line DNA Methylation* Embryonic Stem Cells / cytology, physiology* Gene Expression Profiling / standards* Humans Induced Pluripotent Stem Cells / cytology, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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U01 ES017155-04/ES/NIEHS NIH HHS; U01ES017155/ES/NIEHS NIH HHS |
| Comments/Corrections | |
Comment In:
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Nat Methods. 2011 Apr;8(4):287
[PMID:
21574272
]
Nat Rev Genet. 2011 Mar;12(3):153 [PMID: 21331086 ] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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