Document Detail

Refeeding-induced expression of neuronal nitric oxide synthase in the rat paraventricular nucleus.
MedLine Citation:
PMID:  15925328     Owner:  NLM     Status:  MEDLINE    
We have previously reported that food deprivation decreases the expression of neuronal nitric oxide synthase (nNOS) in the hypothalamic paraventricular nucleus (PVN) of rats, and this reduction is inhibited by blockade of glucocorticoid receptors. In this study, we examined whether the fasting-induced decrease in nNOS gene expression in the PVN is restored by refeeding. The number of nNOS immunopositive cells in the PVN, which was markedly decreased by 48 h of food deprivation, increased significantly after 6 h of refeeding and was fully restored by 24 h after refeeding. The plasma corticosterone level, which was markedly increased by food deprivation, decreased significantly within 30 min after refeeding and returned to the free fed control level by 6 h. Synthetic glucocorticoid dexamethasone blocked the refeeding-induced nNOS expression in the PVN without suppressing food intake. Refeeding with a non-caloric food mash for 5 h failed to restore the fasting-induced decrease in the PVN-nNOS but did, however, successfully restore the plasma corticosterone level. These results suggest that the refeeding-induced nNOS expression in the PVN is a nutrient-directed event and that plasma glucocorticoids may play an inhibitory role in the regulatory pathway. Additionally, glucocorticoid disinhibition alone does not appear to be sufficient to induce nNOS expression in the PVN; nNOS expression in the PVN upon refeeding may require both nutrient supplementation and glucocorticoid disinhibition.
Jeong Won Jahng; Joo Young Lee; Sang Bae Yoo; Yun Mi Kim; Vitaly Ryu; Dong Won Kang; Jong-Ho Lee
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  1048     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-17     Completed Date:  2005-09-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  185-92     Citation Subset:  IM    
Department of Pharmacology, BK21 project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, South Korea.
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MeSH Terms
Analysis of Variance
Antiemetics / pharmacology
Behavior, Animal
Corticosterone / blood
Cyclic AMP Response Element-Binding Protein / metabolism
Dexamethasone / pharmacology
Feeding Behavior / drug effects,  physiology*
Food Deprivation / physiology*
Gene Expression Regulation, Enzymologic / drug effects,  physiology*
Immunohistochemistry / methods
Nerve Tissue Proteins / metabolism*
Nitric Oxide Synthase / metabolism*
Nitric Oxide Synthase Type I
Paraventricular Hypothalamic Nucleus / drug effects,  enzymology*
Rats, Sprague-Dawley
Time Factors
Reg. No./Substance:
0/Antiemetics; 0/Cyclic AMP Response Element-Binding Protein; 0/Nerve Tissue Proteins; 50-02-2/Dexamethasone; 50-22-6/Corticosterone; EC Oxide Synthase; EC Oxide Synthase Type I; EC protein, rat

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