Document Detail


Reevaluation of assay methods and establishment of kit for superoxide dismutase activity.
MedLine Citation:
PMID:  6099057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Superoxide dismutase (SOD) activity was measured by seven assay methods. The nitrite method was found to be the best for our SOD assay kit. This method was then modified to give better sensitivity and minimize interference by coexisting protein, a factor which has been previously ignored. Hydroxylamine or its O-sulfonic acid, xanthine oxidase, hypoxanthine, EDTA, and the sample were incubated with or without KCN at pH 8.2, 37 degrees C, for 30 min. Diazo dye-forming reagent was added and the absorption was measured at 550 nm. Human plasma and erythrocyte lysate from healthy adults and Down's syndrome patients were assayed by this SOD kit and by the cytochrome c method. Our kit gave 8.5 times higher sensitivity than the cytochrome c method. This high sensitivity allowed the use of a simple spectrophotometer and, moreover, only one dilution was needed to determine the SOD unit with the help of our formulas. Good recovery, reproducibility, and stability of reagents were demonstrated.
Authors:
Y Oyanagui
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  142     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1985-03-29     Completed Date:  1985-03-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  290-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Chemical Phenomena
Chemistry
Child
Cytochrome c Group
Erythrocytes / enzymology
Female
Humans
Male
Rats
Reagent Kits, Diagnostic*
Serum Albumin, Bovine
Superoxide Dismutase / analysis*,  blood
Chemical
Reg. No./Substance:
0/Cytochrome c Group; 0/Reagent Kits, Diagnostic; 0/Serum Albumin, Bovine; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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