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Reemergence of chikungunya virus in bo, sierra leone.
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PMID:  23764023     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
We diagnosed 400 possible IgM-positive cases of chikungunya virus in Bo, Sierra Leone, during July 2012-January 2013 by using lateral flow immunoassays. Cases detected likely represent only a small fraction of total cases. Further laboratory testing is required to confirm this outbreak and characterize the virus.
Authors:
Rashid Ansumana; Kathryn H Jacobsen; Tomasz A Leski; Andrea L Covington; Umaru Bangura; Mary H Hodges; Baochuan Lin; Alfred S Bockarie; Joseph M Lamin; Moses J Bockarie; David A Stenger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Emerging infectious diseases     Volume:  19     ISSN:  1080-6059     ISO Abbreviation:  Emerging Infect. Dis.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508155     Medline TA:  Emerg Infect Dis     Country:  United States    
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Languages:  eng     Pagination:  1108-10     Citation Subset:  IM    
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Journal Information
Journal ID (nlm-ta): Emerg Infect Dis
Journal ID (iso-abbrev): Emerging Infect. Dis
Journal ID (publisher-id): EID
ISSN: 1080-6040
ISSN: 1080-6059
Publisher: Centers for Disease Control and Prevention
Article Information

Print publication date: Month: 7 Year: 2013
Volume: 19 Issue: 7
First Page: 1108 Last Page: 1110
PubMed Id: 23764023
ID: 3713974
Publisher Id: 12-1563
DOI: 10.3201/eid1907.121563

Reemergence of Chikungunya Virus in Bo, Sierra Leone Alternate Title:Chikungunya Virus in Sierra Leone
Rashid Ansumana
Kathryn H. Jacobsen
Tomasz A. Leski
Andrea L. Covington
Umaru Bangura
Mary H. Hodges
Baochuan Lin
Alfred S. Bockarie
Joseph M. Lamin
Moses J. Bockarie
David A. Stenger
Njala University, Bo, Sierra Leone (R. Ansumana, A.S. Bockarie);
Mercy Hospital Bo (R. Ansumana, A.L. Covington, U. Bangura, A.S. Bockarie, J.M. Lamin);
Liverpool School of Tropical Medicine, Liverpool, United Kingdom (R. Ansumana, M.H. Hodges, M.J. Bockarie);
George Mason University, Fairfax, Virginia, USA (K.H. Jacobsen);
US Naval Research Laboratory, Washington, DC, USA (T.A. Leski, B. Lin, D.A. Stenger)
Correspondence: Address for correspondence: Kathryn H. Jacobsen, Department of Global and Community Health, George Mason University, 4400 University Dr, Mailstop 5B7, Fairfax, VA 22030, USA; email: kjacobse@gmu.edu

Outbreaks of infection with chikungunya virus (CHIKV), an alphavirus that is transmitted by bites of infected Aedes spp. mosquitoes, were frequent in sub-Saharan Africa and southern and Southeast Asia during the 1950s–1970s, but the infection largely disappeared in the 1980s; only sporadic cases were observed (1). The virus reemerged in the early 2000s; major outbreaks were reported in Kenya, some island nations in the Indian Ocean, and several countries in Asia (2,3).

The primary symptoms of CHIKV infection are high fever (>38.5°C [102°F]) and severe pain in the distal joints of the extremities or the lumbar spine. A maculopapular rash, sensorineural impairment, severe headache, and other nonspecific symptoms may also occur. Symptoms usually resolve within 1–2 weeks after onset of fever, but for a sizeable proportion of patients, arthralgia and arthritis become chronic and pain persists for years (2,3).

A nationwide serosurvey in Sierra Leone in 1972 detected cases of CHIKV infection throughout the country (4), but we are not aware of any cases reported since the mid-1970s. Two recent developments made reemergence appear imminent. First, outbreaks of reemerging CHIKV have been reported in neighboring Guinea (5) and in Senegal (6). Second, recent yellow fever cases in Sierra Leone have shown that Aedes spp. mosquito–borne infections are common (7). Thus, it was not surprising when we initiated an infectious disease surveillance study in July 2012 in the city of Bo, in Southern Province, Sierra Leone, that we detected possible chikungunya virus infections. We report initial results of our investigation.


The Study

On July 7, 2012, the Mercy Hospital Research Laboratory (MHRL) in Bo, Sierra Leone, initiated a 1-year infectious disease surveillance program to identify the diversity of pathogens causing febrile illness in the city. A tiered analysis approach was used. First, all specimens from febrile study participants were tested for ≈12 infections with various pathogens, including CHIKV, by commercially available test kits. Specimens that showed negative results in this first round of testing were further tested by using cultures, multiplex PCR, and resequencing pathogen microarrays. The research protocol was approved by Njala University, George Mason University, the Liverpool School of Tropical Medicine, the US Naval Research Laboratory, and the Sierra Leone Ethics and Scientific Review Committee.

During July 7, 2012–January 10, 2013, MHRL conducted first-tier lateral flow immunoassay (LFI) tests of blood samples from all 932 outpatients ≥5 years of age who had been clinically examined at the hospital, were found to have febrile illness, and consented to having blood drawn for laboratory testing. LFI test kits (SD Bioline; Standard Diagnostics, Inc., Seoul, South Korea) were used for diagnosis of IgM against CHIKV; IgG and IgM against dengue virus and hepatitis A virus; hepatitis B virus surface antigen, hepatitis C virus, HIV-1/2, and antibodies against these viruses; and IgG and IgM against Leptospira spp., Salmonella enterica Serovar typhi, and syphilis.

Most patients reported that they had sought medical care within several days after the onset of their febrile illnesses. Levels of IgM against CHIKV are usually detectable by immunochromatographic methods within a few days after infection and persist for ≈3–4 months (1,2). The LFI test kits for CHIKV were reported by the manufacturer to have a sensitivity of 97.1% and a specificity of 91.1% compared with those of ELISA (8). An independent evaluation found a sensitivity of 50.8% and a specificity of 89.2% for the kits; sensitivity ranged from 40.9% 1–5 days after onset of illness to 65.4% 16–20 days after onset (9). Specificity decreases after the first week (10).

More than half of the cases tested during the first week of the surveillance program were positive by LFI for CHIKV. Thus, we notified the Sierra Leone Ministry of Health and Sanitation of a possible CHIKV outbreak. By January 10, 2013, 400 (42.9%) of 932 febrile patients were positive by LFI for CHIKV (Figure 1). Ages of the 400 CHIKV IgM-positive patients ranged from 6 years to 85 years; 172 (43.0%) were male patients. Of these 400 patients, 220 (55.0%) reported arthralgia, 189 (47.3%) chills, and 156 (39.0%) headaches. Co-infections were common; 92 (23.0%.) were co-infected with malaria, 37 (9.3%) with HIV, 33 (8.3%) with hepatitis B virus, and smaller numbers with hepatitis A, hepatitis C, tuberculosis, typhoid, and syphilis. Four CHIKV-positive samples were also positive for dengue.

On July 28, MHRL launched an Ushahidi-based website (www.ushahidi.com) to compile case reports. Details about the patients who were positive for CHIKV were uploaded to the MHRL website (www.mhrlsl.com/GIA/ushahidi) and, if possible, were geolocated on an open street map (www.openstreetmap.org) that linked to a map of Bo created by MHRL for health research purposes (11). The map showed that the cases were located throughout Bo (Figure 2). Of the 400 LFI-positive case-patients, 319 (79.8%) could be mapped; the remainder did not provide a home street address on the laboratory patient information form. However, the sample was not population-based because Mercy Hospital is 1 of several hospitals serving Bo, so a city-wide attack rate could not be determined.

Results of attempts by the US Naval Research Laboratory to confirm the LFI results by using semi-nested reverse transcription PCR on fast technique for analysis of nucleic acid–preserved samples were inconclusive, possibly because of genetic sequence variation from well-characterized strains or because of the timing of specimen collections. Viral loads for humans with CHIKV infection decrease after the second day of symptoms, and viral titers may be low after the fifth day (12,13). Because CHIKV nucleic acids are only detectable in serum for a few days, reverse transcription PCR results are often discordant with those of serologic (IgM and IgG) assays. Confirmation that an outbreak occurred is dependent on isolation of the virus, followed by molecular characterization, full-genome sequencing, and phylogenetic mapping.


Conclusions

Our results suggest that an outbreak of chikungunya virus occurred in Sierra Leone. The exact time of the reemergence of this virus cannot be pinpointed, but retrospective analyses of outpatient charts suggested that, on the basis of syndromic criteria, the first cases occurred in January 2012 and the outbreak peaked during the rainy season in 2012. Other outbreaks reported in central and west Africa have also occurred during the rainy season, which is typical for Aedes spp. mosquito–borne infections (6,14,15). Because Mercy Hospital serves only a relatively small proportion of the residents of Bo, the cases detected likely represent only a small fraction of the total cases that have occurred. Further study will be required to confirm the laboratory results and, if further investigation is warranted, to document the extent of the outbreak.


Notes

Suggested citation for this article: Ansumana R, Jacobsen KH, Leski TA, Covington AL, Bangura U, Hodges MH, et al. Reemergence of chikungunya virus in Bo, Sierra Leone. Emerg Infect Dis [Internet]. 2013 Jul [date cited]. http://dx.doi.org/10.3201/eid1907.121563

Acknowledgments

We thank Shalinee Bhoobun, Alhaji Alusine Jalloh, Alfred Momoh, Jacob A.G. Buanie, and Abdulai Gad Swarray for contributions to the study.

This study was supported by the Defense Threat Reduction Agency (Fort Belvoir, Virginia, USA).

Mr Ansumana is a director of the Mercy Hospital Research Laboratory in Bo, Sierra Leone, and a PhD candidate at the Liverpool School of Tropical Medicine. His research interests include nonmalarial infectious diseases and the use of information and communications technology for research in resource-limited settings.


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Figures

[Figure ID: F1]
Figure 1 

Weekly number of IgM-positive chikungunya virus test results at Mercy Hospital Research Laboratory, Bo, Sierra Leone, July 7, 2012–January 4, 2013.



[Figure ID: F2]
Figure 2 

Residence locations for IgM-positive cases of infection with chikungunya virus, Bo, Sierra Leone, July 7, 2012–January 4, 2013.



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Keywords: Keywords: chikungunya virus, CHIKV, viruses, reemerging infectious disease, Sierra Leone, Africa.

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