Document Detail


Reductive degradation of pyrazine-2-carboxylate by a newly isolated Stenotrophomonas sp. HCU1.
MedLine Citation:
PMID:  20217461     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A bacterium growing on pyrazine-2-carboxylate broth was isolated, purified and identified as a strain of Stenotrophomonas sp. based on polyphasic taxonomic analyses and designated as strain HCU1. 16S rRNA gene sequence of strain HCU1 showed 98.7% sequence similarity with the type strain of Stenotrophomonas maltophilia belonging to Gammaproteobacteria. Growth of strain HCU1 was demonstrated when pyrazine-2-carboxylate was used as a sole source of nitrogen. Ring reduction of pyrazine-2-carboxylate was shown as increase in absorbance at 268 nm and the reduced product was confirmed as 1,2,5,6-tetrahydropyrazine-2-carboxylate, while a ring opened product, 2-amino-2-hydroxy-3-(methylamino) propanoic acid (with a loss in carbon atom), indicated a reductive degradation of pyrazine-2-carboxylate by strain HCU1.
Authors:
K S Rajini; Ch Sasikala; Ch V Ramana
Publication Detail:
Type:  Journal Article     Date:  2010-03-10
Journal Detail:
Title:  Biodegradation     Volume:  21     ISSN:  1572-9729     ISO Abbreviation:  Biodegradation     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-04     Completed Date:  2010-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9100834     Medline TA:  Biodegradation     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  801-13     Citation Subset:  IM    
Affiliation:
Department of Plant Sciences, University of Hyderabad, Hyderabad, India. keerthirajini5@yahoo.co.in
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bacterial Proteins / chemistry
Biodegradation, Environmental
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Enzyme Assays
Kinetics
Molecular Sequence Data
Molecular Weight
Oxidation-Reduction
Peptides / chemistry
Phylogeny
Pyrazines / chemistry,  metabolism*
RNA, Ribosomal, 16S / genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectroscopy, Fourier Transform Infrared
Stenotrophomonas / classification,  genetics,  growth & development,  isolation & purification*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Peptides; 0/Pyrazines; 0/RNA, Ribosomal, 16S; 0/pyrazine-2-carboxylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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