| Reduction of viral load and immune complex load on CD4+ lymphocytes as a consequence of highly active antiretroviral treatment (HAART) in HIV-infected hemophilia patients. | |
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MedLine Citation:
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PMID: 10482364 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVES: Human immunodeficiency virus (HIV)-induced immune complex load on circulating CD4+ blood lymphocytes is associated with dysfunction and depletion of CD4+ lymphocytes and with increased monocyte/macrophage function. It was investigated whether HAART reduces both the viral load in plasma and the number of immune complex-coated CD4+ lymphocytes in the blood, and whether CD4+ counts are associated with viral load and/or immune complex load. MATERIALS AND METHODS: Twelve HIV+ hemophilia patients before and after conversion to HAART (group 1); eight HIV+ hemophilia patients without antiretroviral therapy (group 2). HIV-1 RNA copies in plasma using NASBA/Nuclisens kits; CD4+ lymphocytes coated in-vivo with immune complexes using flowcytometry on whole blood samples; in-vitro responses of immune complex-coated T lymphocytes in cell culture assays. RESULTS: After conversion to HAART there was a significant reduction of viral load, CD4+ gp120+, CD4+ IgM+, and CD4+ IgG+ circulating blood lymphocytes and plasma neopterin, paralleled by a significant increase of CD4+ and CD8+ counts. The percentage of immune complex-coated CD4+ lymphocytes of converted patients was significantly associated with CD4+ counts, in-vitro responses to concanavalin A (Con A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA), anti-CD3 and pooled allogeneic stimulator cells, and with plasma neopterin levels. CONCLUSION: HAART reduces viral load and HIV-induced immune complex load on circulating CD4+ blood lymphocytes. The results of this study can be interpreted to suggest that HAART increases CD4+ lymphocyte counts in part by counteracting HIV-induced autoimmune phenomena. |
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Authors:
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V Daniel; C Süsal; A Melk; R Weimer; M Kröpelin; R Zimmermann; A Huth-Kühne; C Uhle; G Opelz |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Immunology letters Volume: 69 ISSN: 0165-2478 ISO Abbreviation: Immunol. Lett. Publication Date: 1999 Aug |
Date Detail:
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Created Date: 1999-10-21 Completed Date: 1999-10-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7910006 Medline TA: Immunol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 283-9 Citation Subset: IM; X |
Affiliation:
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Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Germany. volker_daniel@med.uni-heidelberg.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-HIV Agents
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pharmacology*,
therapeutic use Antibodies, Anti-Idiotypic / blood*, immunology Antigen-Antibody Complex / blood* Autoantibodies / blood*, immunology Autoimmunity CD4-Positive T-Lymphocytes / immunology*, virology Concanavalin A / pharmacology Drug Therapy, Combination HIV Envelope Protein gp120 / blood HIV Infections / blood, complications, drug therapy, immunology* HIV Protease Inhibitors / pharmacology*, therapeutic use Hemophilia A / blood, complications, immunology* Humans Immunoglobulins / blood* Lymphocyte Activation / drug effects Lymphocyte Culture Test, Mixed Male Mitogens / pharmacology RNA, Viral / blood Reverse Transcriptase Inhibitors / pharmacology*, therapeutic use Viral Load Viremia / drug therapy*, immunology |
| Chemical | |
Reg. No./Substance:
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0/Anti-HIV Agents; 0/Antibodies, Anti-Idiotypic; 0/Antigen-Antibody Complex; 0/Autoantibodies; 0/HIV Envelope Protein gp120; 0/HIV Protease Inhibitors; 0/Immunoglobulins; 0/Mitogens; 0/RNA, Viral; 0/Reverse Transcriptase Inhibitors; 11028-71-0/Concanavalin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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