Document Detail


Reduction of viral load and immune complex load on CD4+ lymphocytes as a consequence of highly active antiretroviral treatment (HAART) in HIV-infected hemophilia patients.
MedLine Citation:
PMID:  10482364     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: Human immunodeficiency virus (HIV)-induced immune complex load on circulating CD4+ blood lymphocytes is associated with dysfunction and depletion of CD4+ lymphocytes and with increased monocyte/macrophage function. It was investigated whether HAART reduces both the viral load in plasma and the number of immune complex-coated CD4+ lymphocytes in the blood, and whether CD4+ counts are associated with viral load and/or immune complex load. MATERIALS AND METHODS: Twelve HIV+ hemophilia patients before and after conversion to HAART (group 1); eight HIV+ hemophilia patients without antiretroviral therapy (group 2). HIV-1 RNA copies in plasma using NASBA/Nuclisens kits; CD4+ lymphocytes coated in-vivo with immune complexes using flowcytometry on whole blood samples; in-vitro responses of immune complex-coated T lymphocytes in cell culture assays. RESULTS: After conversion to HAART there was a significant reduction of viral load, CD4+ gp120+, CD4+ IgM+, and CD4+ IgG+ circulating blood lymphocytes and plasma neopterin, paralleled by a significant increase of CD4+ and CD8+ counts. The percentage of immune complex-coated CD4+ lymphocytes of converted patients was significantly associated with CD4+ counts, in-vitro responses to concanavalin A (Con A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA), anti-CD3 and pooled allogeneic stimulator cells, and with plasma neopterin levels. CONCLUSION: HAART reduces viral load and HIV-induced immune complex load on circulating CD4+ blood lymphocytes. The results of this study can be interpreted to suggest that HAART increases CD4+ lymphocyte counts in part by counteracting HIV-induced autoimmune phenomena.
Authors:
V Daniel; C Süsal; A Melk; R Weimer; M Kröpelin; R Zimmermann; A Huth-Kühne; C Uhle; G Opelz
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Immunology letters     Volume:  69     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-10-21     Completed Date:  1999-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  283-9     Citation Subset:  IM; X    
Affiliation:
Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Germany. volker_daniel@med.uni-heidelberg.de
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MeSH Terms
Descriptor/Qualifier:
Anti-HIV Agents / pharmacology*,  therapeutic use
Antibodies, Anti-Idiotypic / blood*,  immunology
Antigen-Antibody Complex / blood*
Autoantibodies / blood*,  immunology
Autoimmunity
CD4-Positive T-Lymphocytes / immunology*,  virology
Concanavalin A / pharmacology
Drug Therapy, Combination
HIV Envelope Protein gp120 / blood
HIV Infections / blood,  complications,  drug therapy,  immunology*
HIV Protease Inhibitors / pharmacology*,  therapeutic use
Hemophilia A / blood,  complications,  immunology*
Humans
Immunoglobulins / blood*
Lymphocyte Activation / drug effects
Lymphocyte Culture Test, Mixed
Male
Mitogens / pharmacology
RNA, Viral / blood
Reverse Transcriptase Inhibitors / pharmacology*,  therapeutic use
Viral Load
Viremia / drug therapy*,  immunology
Chemical
Reg. No./Substance:
0/Anti-HIV Agents; 0/Antibodies, Anti-Idiotypic; 0/Antigen-Antibody Complex; 0/Autoantibodies; 0/HIV Envelope Protein gp120; 0/HIV Protease Inhibitors; 0/Immunoglobulins; 0/Mitogens; 0/RNA, Viral; 0/Reverse Transcriptase Inhibitors; 11028-71-0/Concanavalin A

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