Document Detail


Reduction of regulatory T cells in skin lesions but not in peripheral blood of patients with systemic scleroderma.
MedLine Citation:
PMID:  21097800     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine the frequency and suppressive capacity of regulatory T cells (T(reg)) and their association with clinical parameters in patients with systemic scleroderma (SSc).
METHODS: Peripheral blood from 25 patients with SSc, 15 patients with localised scleroderma (LS) and 29 healthy controls (HC) was studied. Analysis of CD4(+) forkhead box P3 (Foxp3)(+) and CD4(+)CD25(++)Foxp3(+) T(reg) subpopulations was carried out by flow cytometry and cell proliferation was quantified by (3)H-thymidine incorporation. Quantitative analysis of T(reg) was further performed in skin biopsies from 17 patients with SSc and 21 patients with LS using anti-CD4 and anti-Foxp3 monoclonal antibodies for immunohistochemistry.
RESULTS: The frequency of CD4(+)Foxp3(+) and CD4(+)CD25(++)Foxp3(+) T(reg) in peripheral blood from patients with SSc was not significantly different from that of patients with LS or HC. The suppressive capacity of CD4(+)CD25(++) T(reg) in SSc was also found to be similar to that of HC. Phenotypic and functional data revealed no significant difference between the limited or diffuse form of SSc. Moreover, therapy with bosentan showed no significant effect on the frequency of T(reg) during the course of the disease. However, the frequency of T(reg) in skin lesions from patients with SSc or LS, determined as the percentage of CD4(+) cells expressing Foxp3 in the inflammatory infiltrate, was significantly reduced compared with other inflammatory skin diseases.
CONCLUSION: These results indicate that although the authors found no defect in the frequency or function of peripheral T(reg) subpopulations, the reduction of CD4(+)Foxp3(+) T(reg) in the skin of patients with SSc may be important in the pathogenesis of the disease.
Authors:
S Klein; C C Kretz; V Ruland; C Stumpf; M Haust; W Hartschuh; M Hartmann; A Enk; E Suri-Payer; N Oberle; P H Krammer; A Kuhn
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-19
Journal Detail:
Title:  Annals of the rheumatic diseases     Volume:  70     ISSN:  1468-2060     ISO Abbreviation:  Ann. Rheum. Dis.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-06-30     Completed Date:  2011-11-18     Revised Date:  2013-06-18    
Medline Journal Info:
Nlm Unique ID:  0372355     Medline TA:  Ann Rheum Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  1475-81     Citation Subset:  IM    
Affiliation:
Division of Immunogenetics, Tumorimmunology Program, German Cancer Research Center, Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biopsy
CD4-Positive T-Lymphocytes / immunology
Case-Control Studies
Dermatitis / immunology
Dermatologic Agents / pharmacology,  therapeutic use
Female
Forkhead Transcription Factors / analysis
Humans
Immune Tolerance / immunology
Male
Middle Aged
Receptor, Endothelin A / antagonists & inhibitors
Scleroderma, Localized / immunology
Scleroderma, Systemic / drug therapy,  immunology*,  pathology
Skin / immunology*,  pathology
Sulfonamides / pharmacology,  therapeutic use
T-Lymphocyte Subsets / immunology
T-Lymphocytes, Regulatory / drug effects,  immunology*
Chemical
Reg. No./Substance:
0/Dermatologic Agents; 0/FOXP3 protein, human; 0/Forkhead Transcription Factors; 0/Receptor, Endothelin A; 0/Sulfonamides; Q326023R30/bosentan

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