Document Detail

Reduction of reequilibration time following gradient elution reversed-phase liquid chromatography.
MedLine Citation:
PMID:  2301727     Owner:  NLM     Status:  MEDLINE    
A simple and convenient method for the reduction of column reequilibration time following gradient elution reversed-phase liquid chromatography is described. This method utilizes the addition of a constant volume of 3% 1-propanol to the mobile phase throughout the solvent gradient to provide consistent solvation of the reversed-phase stationary phase. Reductions in reequilibration time of up to 78% have been observed. The effect of alkyl chain bonding density on reequilibration volume is also examined. A maximum in the mobile phase volume necessary to reequilibrate the column is found at a bonding density of about 2.9 mumol/m2. The relationship of reequilibration volume to bonding density supports the partitioning model of retention for reversed-phase liquid chromatography.
L A Cole; J G Dorsey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  62     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-03-07     Completed Date:  1990-03-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  16-21     Citation Subset:  IM    
Department of Chemistry, University of Florida, Gainesville 32611.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
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