Document Detail

Reduction of reaction differences between human mercaptalbumin and human nonmercaptalbumin measured by the bromcresol purple method.
MedLine Citation:
PMID:  10556654     Owner:  NLM     Status:  MEDLINE    
Although the bromcresol purple (BCP) method provides high specificity in measurements of serum albumin concentrations, we discovered a reaction difference between the values for human mercaptalbumin (HMA) and human nonmercaptalbumin (HNA) measured by the BCP. We found that the color intensity of HMA with BCP present in the reduced form in the albumin of fresh serum, was lower than for HNA. While maintaining specificity for albumin, we reduced the reaction difference between HMA and HNA, with the addition of sodium dodecylsulfate and 5,5'-dithiobis(2-nitrobenzoic acid) to the BCP reagent. The mean albumin concentration by the BCP procedure for 63 fresh sera and the 63 sera stored for 2 days at room temperature were 35.6 g/L and 38.1 g/L, respectively. Those by the modified BCP procedure were 39.8 g/L and 39.9 g/L, respectively. The difference in measured values between the fresh and stored sera which is believed to be caused by the conversion of HMA to HNA during the storage of sera was not observed in the case of the modified BCP procedure. Our modified BCP method is effective in eliminating uncertainty of the albumin concentration assigned to assay calibrators for the conventional BCP method.
Y Muramoto; M Matsushita; T Irino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  289     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-01-11     Completed Date:  2000-01-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-78     Citation Subset:  IM    
Department of Central Clinical Laboratory, Cardiovascular Institute, 7-3-10, Roppongi, Minato-ku, Tokyo, Japan.
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MeSH Terms
Blood Chemical Analysis / methods*
Blood Specimen Collection
Bromcresol Green / chemistry
Bromcresol Purple / chemistry
Indicators and Reagents / chemistry
Kidney Failure / blood
Linear Models
Sensitivity and Specificity
Serum Albumin / analysis*,  chemistry
Spectrum Analysis / methods
Time Factors
Reg. No./Substance:
0/Indicators and Reagents; 0/Serum Albumin; 0/mercaptoalbumin; 0/nonmercaptalbumin; 115-40-2/Bromcresol Purple; 76-60-8/Bromcresol Green

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