| Reduction of reaction differences between human mercaptalbumin and human nonmercaptalbumin measured by the bromcresol purple method. | |
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MedLine Citation:
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PMID: 10556654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although the bromcresol purple (BCP) method provides high specificity in measurements of serum albumin concentrations, we discovered a reaction difference between the values for human mercaptalbumin (HMA) and human nonmercaptalbumin (HNA) measured by the BCP. We found that the color intensity of HMA with BCP present in the reduced form in the albumin of fresh serum, was lower than for HNA. While maintaining specificity for albumin, we reduced the reaction difference between HMA and HNA, with the addition of sodium dodecylsulfate and 5,5'-dithiobis(2-nitrobenzoic acid) to the BCP reagent. The mean albumin concentration by the BCP procedure for 63 fresh sera and the 63 sera stored for 2 days at room temperature were 35.6 g/L and 38.1 g/L, respectively. Those by the modified BCP procedure were 39.8 g/L and 39.9 g/L, respectively. The difference in measured values between the fresh and stored sera which is believed to be caused by the conversion of HMA to HNA during the storage of sera was not observed in the case of the modified BCP procedure. Our modified BCP method is effective in eliminating uncertainty of the albumin concentration assigned to assay calibrators for the conventional BCP method. |
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Authors:
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Y Muramoto; M Matsushita; T Irino |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 289 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1999 Nov |
Date Detail:
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Created Date: 2000-01-11 Completed Date: 2000-01-11 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 69-78 Citation Subset: IM |
Affiliation:
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Department of Central Clinical Laboratory, Cardiovascular Institute, 7-3-10, Roppongi, Minato-ku, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Blood Chemical Analysis / methods* Blood Specimen Collection Bromcresol Green / chemistry Bromcresol Purple / chemistry Humans Indicators and Reagents / chemistry Kidney Failure / blood Linear Models Oxidation-Reduction Sensitivity and Specificity Serum Albumin / analysis*, chemistry Spectrum Analysis / methods Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Serum Albumin; 0/mercaptoalbumin; 0/nonmercaptalbumin; 115-40-2/Bromcresol Purple; 76-60-8/Bromcresol Green |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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