Document Detail


Reduction of miR-21 induces glioma cell apoptosis via activating caspase 9 and 3.
MedLine Citation:
PMID:  20514462     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extensive data indicate that miR-21 plays a critical role in gliomagenesis, however, knowledge is limited on the mechanism of action of miR-21, including cell proliferation, apoptosis, and migration. In this study, we showed that down-regulation of miR-21 expression by antisense oligonucleotides inhibited glioma cell proliferation and induced cell apoptosis. Moreover, reduction of miR-21 activated caspase 9 and 3, which may be mediated by modulating multiple potential target genes, such as TIMP3. Together, these findings indicate that miR-21 plays a key role in regulating cell apoptosis in gliomas and may serve as a target for effective therapies.
Authors:
Xuan Zhou; Junxia Zhang; Qiang Jia; Yu Ren; Yingyi Wang; Lei Shi; Ning Liu; Guangxiu Wang; Peiyu Pu; Yongping You; Chunsheng Kang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  24     ISSN:  1791-2431     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-01     Completed Date:  2010-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  195-201     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Tianjin Medical University General Hospital, Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*,  genetics
Brain Neoplasms / drug therapy,  genetics,  metabolism,  pathology*
Caspase 3 / metabolism*
Caspase 9 / metabolism*
Cell Line, Tumor
Down-Regulation / drug effects,  physiology
Enzyme Activation / drug effects
Gene Expression Regulation, Neoplastic / drug effects
Glioma / drug therapy,  genetics,  metabolism,  pathology*
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / antagonists & inhibitors*,  genetics
Oligodeoxyribonucleotides, Antisense / pharmacology*,  therapeutic use
Xenograft Model Antitumor Assays
Chemical
Reg. No./Substance:
0/MIRN21 microRNA, human; 0/MicroRNAs; 0/Oligodeoxyribonucleotides, Antisense; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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