Document Detail

Reduction of lipid hydroperoxides by apolipoprotein B-100.
MedLine Citation:
PMID:  10334937     Owner:  NLM     Status:  MEDLINE    
We have previously isolated two proteins which can reduce phosphatidylcholine hydroperoxide (PC-OOH) from human blood plasma and identified one of the proteins as apolipoprotein A-I (Mashima, R. , et al. (1998) J. Lipid Res. 39, 1133-1140). In the present study we have identified the other protein as apolipoprotein B-100 (apo B-100) by amino acid sequence analysis of its tryptic peptides. The reactivity of lipid hydroperoxides with apo B-100 decreased in the order of PC-OOH > linoleic acid hydroperoxide > cholesteryl ester hydroperoxide under our experimental conditions. Pretreatment of apo B-100 with chloramine T, an oxidant of methionine, diminished the PC-OOH-reducing activity, indicating that some of 78 methionines are responsible for the reduction of PC-OOH. Despite the presence of 6 methionines in albumin, albumin was inactive to reduce PC-OOH. Free methionine was also inactive. These data suggest that the accessibility and binding of lipid hydroperoxides to the protein methionine residues are crucial for reduction of lipid hydroperoxides.
R Mashima; S Yoshimura; Y Yamamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  259     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-06-25     Completed Date:  1999-06-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  185-9     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan.
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MeSH Terms
Apolipoprotein B-100
Apolipoproteins B / blood*
Blood Proteins / chemistry
Chloramines / pharmacology
Lipid Peroxides / metabolism*
Methionine / analogs & derivatives,  chemistry
Phosphatidylcholines / blood,  metabolism
Tosyl Compounds / pharmacology
Reg. No./Substance:
0/Apolipoprotein B-100; 0/Apolipoproteins B; 0/Blood Proteins; 0/Chloramines; 0/Lipid Peroxides; 0/Phosphatidylcholines; 0/Tosyl Compounds; 0/phosphatidylcholine hydroperoxide; 127-65-1/chloramine-T; 454-41-1/methionine sulfoxide; 63-68-3/Methionine

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