Document Detail


Reduction of iodonitrotetrazolium violet by superoxide radicals.
MedLine Citation:
PMID:  2829896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
p-Iodonitrotetrazolium (2-(4-iodophenyl-3-(4-nitrophenyl)- 5-phenyltetrazolium; INT) was reduced to a water-soluble product with an absorbance maxima at about 505 nm (reddish pink) by superoxide anion (O2-.) generated by xanthine/xanthine oxidase. The rate of INT reduction was linearly related to the xanthine oxidase activities, and was inhibited by superoxide dismutase. The soluble product may further be converted to an insoluble product, presumably nonionic formazan, with an absorbance maxima of 490 nm (purplish), under certain conditions, and the rate of the formazan formation depended on pH and protein concentration.
Authors:
J J Podczasy; R Wei
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  150     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-03-23     Completed Date:  1988-03-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1294-301     Citation Subset:  IM    
Affiliation:
Chemistry Department, Cleveland State University, Ohio 44115.
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MeSH Terms
Descriptor/Qualifier:
Free Radicals
Hydrogen-Ion Concentration
Kinetics
Oxidation-Reduction
Proteins / pharmacology
Spectrophotometry
Superoxide Dismutase / pharmacology
Superoxides* / metabolism
Tetrazolium Salts* / metabolism
Xanthine
Xanthine Oxidase / metabolism
Xanthines / metabolism
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Proteins; 0/Tetrazolium Salts; 0/Xanthines; 11062-77-4/Superoxides; 146-68-9/iodonitrotetrazolium; 69-89-6/Xanthine; EC 1.15.1.1/Superoxide Dismutase; EC 1.17.3.2/Xanthine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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