| Reduction of iodonitrotetrazolium violet by superoxide radicals. | |
| | |
MedLine Citation:
|
PMID: 2829896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
p-Iodonitrotetrazolium (2-(4-iodophenyl-3-(4-nitrophenyl)- 5-phenyltetrazolium; INT) was reduced to a water-soluble product with an absorbance maxima at about 505 nm (reddish pink) by superoxide anion (O2-.) generated by xanthine/xanthine oxidase. The rate of INT reduction was linearly related to the xanthine oxidase activities, and was inhibited by superoxide dismutase. The soluble product may further be converted to an insoluble product, presumably nonionic formazan, with an absorbance maxima of 490 nm (purplish), under certain conditions, and the rate of the formazan formation depended on pH and protein concentration. |
| | |
Authors:
|
J J Podczasy; R Wei |
Related Documents
:
|
8777016 - Use of edible coatings to preserve quality of lightly (and slightly) processed products. 16688376 - Analysis of consumer cosmetic products for phthalate esters. 10631666 - Characterization of vx on concrete using ion trap secondary ionization mass spectrometry. 3748746 - Effects of exposure to psychophysical scaling on lingual vibrotactile magnitude estimat... 11538466 - Hco emission from h ii-molecular cloud interface regions. 18081206 - Flavan-3-ols: nature, occurrence and biological activity. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 150 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1988 Feb |
Date Detail:
|
Created Date: 1988-03-23 Completed Date: 1988-03-23 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1294-301 Citation Subset: IM |
Affiliation:
|
Chemistry Department, Cleveland State University, Ohio 44115. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Free Radicals Hydrogen-Ion Concentration Kinetics Oxidation-Reduction Proteins / pharmacology Spectrophotometry Superoxide Dismutase / pharmacology Superoxides* / metabolism Tetrazolium Salts* / metabolism Xanthine Xanthine Oxidase / metabolism Xanthines / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Free Radicals; 0/Proteins; 0/Tetrazolium Salts; 0/Xanthines; 11062-77-4/Superoxides; 146-68-9/iodonitrotetrazolium; 69-89-6/Xanthine; EC 1.15.1.1/Superoxide Dismutase; EC 1.17.3.2/Xanthine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cloning of cDNA encoding a new peptide C-terminal alpha-amidating enzyme having a putative membrane-...
Next Document: Expression of the insulinoma gene rig during liver regeneration and in primary cultured hepatocytes.