| Reduction of insulin resistance in HepG2 cells by knockdown of LITAF expression in human THP-1 macrophages. | |
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MedLine Citation:
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PMID: 22282245 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The molecular mechanism by which obesity induces insulin resistance is not completely understood. The aim of this study was to determine how lipopolysaccharide-induced tumor necrosis-α factor (LITAF) influenced obesity-induced insulin resistance using a cellular co-culture system. The cells were divided into 3 groups: palmitic acid (PA) stimulation group, LITAF small interfering RNA (siRNA) group and untreated (NC) group. The LITAF siRNA was used for knockdown of LITAF expression in human THP-1 macrophages. The expression levels of LITAF, IRS-2, IRS-2Tyr465, PI3K, and GLUT2 in each group were measured by using quantitative reverse transcriptase real-time polymerase chain reaction and Western blotting. The expression of LITAF was much higher in the PA group than in the siRNA and NC groups (*P<0.05); meanwhile, the expression of IRS-2, IRS-2Tyr465, PI3K, and GLUT2 was much lower in the PA group than in the NC group (*P<0.05); however, IRS-2, IRS-2Tyr465, PI3K, and GLUT2 had much higher expression in the siRNA group than in the PA group (*P<0.05). It is concluded that PA can induce insulin resistance in liver cells and knockdown of LITAF expression can reduce insulin resistance in liver cells, suggesting LITAF may regulate the insulin signal transduction pathway involved in obesity-induced insulin resistance. |
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Authors:
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Yan Huang; Jie Liu; Yancheng Xu; Zhe Dai; Martins Hedson Alves |
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Publication Detail:
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Type: Journal Article Date: 2012-01-27 |
Journal Detail:
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Title: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban Volume: 32 ISSN: 1672-0733 ISO Abbreviation: J. Huazhong Univ. Sci. Technol. Med. Sci. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101169627 Medline TA: J Huazhong Univ Sci Technolog Med Sci Country: China |
Other Details:
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Languages: eng Pagination: 53-8 Citation Subset: IM |
Affiliation:
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Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China, huangyan17@qq.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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