Document Detail


Reduction of infarct volume and apoptosis by grafting of encapsulated basic fibroblast growth factor-secreting cells in a model of middle cerebral artery occlusion in rats.
MedLine Citation:
PMID:  14705734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECT: This study was conducted to evaluate the effects of grafting encapsulated basic fibroblast growth factor (bFGF)-secreting cells in rat brains subjected to ischemic injury. METHODS: Two cell lines were used for encapsulated grafting in this experiment, namely, a bFGF-secreting cell line established by genetic manipulation of baby hamster kidney (BHK) cells, and a naive BHK cell line. Forty-seven Sprague-Dawley rats were used in this experiment. The animals were divided into the following three groups: those receiving grafts of encapsulated bFGF-secreting cells (BHK-bFGF group); those with grafts of encapsulated naive BHK cells (naive BHK group); and those with no grafts (control group). The authors implanted encapsulated cells into the right striatum of host rats in the BHK-bFGF and naive BHK groups. Six days after grafting, the host and control animals underwent permanent right middle cerebral artery occlusion (MCAO) with an intraluminal suture procedure. The infarct volume was evaluated using 2,3,5-triphenyltetrazolium chloride staining and computerized image analysis 24 hours after MCAO. Fragmentations of DNA in the host brains were analyzed using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling 12 hours after MCAO. The authors found that the infarct volume in the BHK-bFGF group was reduced by approximately 30% compared with that in the naive BHK and control groups. In the ischemic penumbral area, the number of apoptotic cells in the BHK-bFGF group was significantly decreased compared with that in the other groups. CONCLUSIONS: The grafting of encapsulated BHK bFGF-secreting cells protected the brain from ischemic injury. Encapsulation and grafting of genetically engineered cells such as bFGF-secreting cells is thus thought to be a useful method for protection against cerebral ischemia.
Authors:
Kenjiro Fujiwara; Isao Date; Tetsuro Shingo; Hideyuki Yoshida; Kazuki Kobayashi; Akira Takeuchi; Akimasa Yano; Takashi Tamiya; Takashi Ohmoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurosurgery     Volume:  99     ISSN:  0022-3085     ISO Abbreviation:  J. Neurosurg.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-01-06     Completed Date:  2004-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0253357     Medline TA:  J Neurosurg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1053-62     Citation Subset:  AIM; IM    
Affiliation:
Department of Neurological Surgery, Okayama University Medical School, Okayama City, Japan. kenjiro@gb3.so-net.ne.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Brain Infarction / etiology,  pathology*,  prevention & control*
Cell Line
Cell Transplantation*
Cricetinae
Disease Models, Animal
Drug Compounding
Fibroblast Growth Factor 2 / administration & dosage*,  secretion*
Infarction, Middle Cerebral Artery
Male
Rats
Rats, Sprague-Dawley
Transplantation, Heterologous
Chemical
Reg. No./Substance:
103107-01-3/Fibroblast Growth Factor 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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