Document Detail

Reduction in intestinal leukocyte adherence in rat experimental endotoxemia by treatment with the 21-aminosteroid U-74389G.
MedLine Citation:
PMID:  11280645     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To investigate leukocyte adherence in intestinal venules in experimental endotoxemia after treatment with the 21-aminosteroid U-74389G. DESIGN AND SETTING: Prospective, randomized, controlled animal study in an experimental laboratory. SUBJECTS: Twenty-one male Wistar rats weighing 190 +/- 40 g. INTERVENTIONS: The rats were divided equally into three groups: (a) control group, (b) endotoxemia (5 mg/kg lipopolysacharide from Escherichia coli O55:B5), and (c) endotoxemia and U-74389G administration 30 min before (3 mg/kg) and 60 min after endotoxin challenge (1.5 mg/ kg). MEASUREMENTS AND MAIN RESULTS: The distal small intestine of the animals was examined using intravital fluorescence videomicroscopy 2 h after endotoxin challenge. Leukocytes were stained in vivo by means of rhodamine 6G. In the endotoxemic animals we observed a fourfold increase in the count of firmly adherent leukocytes in submucosal post-capillary and collecting venules. Treatment with the 21-aminosteroid U-74389G significantly attenuated the count of sticking leukocytes in the collecting venules (control, 61 +/- 10 cells/mm2; lipopolysaccharide, 237 +/- 42 cells/mm2; U-74389G 125 +/- 9 cells/mm2; p < 0.05). In these venules leukocyte rolling behavior was comparable to that in the control group without endotoxin challenge. CONCLUSIONS: Administration of U-74389G, which has radical scavenging properties, attenuates leukocyte adherence in selected populations of intestinal venules which is found increased during endotoxemia. Thus, 21-aminosteroids may have an impact in the treatment of endotoxin-induced intestinal injury.
C Lehmann; A Georgiew; M Weber; J Birnbaum; W J Kox
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Intensive care medicine     Volume:  27     ISSN:  0342-4642     ISO Abbreviation:  Intensive Care Med     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-03-30     Completed Date:  2001-06-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7704851     Medline TA:  Intensive Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  258-63     Citation Subset:  IM    
Klinik für Anästhesiologie und operative Intensivmedizin, Universitätsklinikum Charité, Humboldt Universität, Berlin, Germany.
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MeSH Terms
Antioxidants / pharmacology*
Cell Adhesion / drug effects*
Endotoxins / toxicity
Free Radicals / metabolism
Hemodynamics / drug effects
Intestine, Small / blood supply,  drug effects
Leukocytes / drug effects*
Mesenteric Veins / drug effects*,  immunology
Microscopy, Fluorescence
Microscopy, Video
Pregnatrienes / pharmacology*
Prospective Studies
Random Allocation
Rats, Wistar
Reactive Oxygen Species / metabolism
Toxemia / drug therapy*,  etiology,  immunology
Reg. No./Substance:
0/Antioxidants; 0/Endotoxins; 0/Free Radicals; 0/Pregnatrienes; 0/Reactive Oxygen Species; 111668-89-4/U 74389F

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