Document Detail

Reduction in bacterial contamination of toothbrushes using the Violight ultraviolet light activated toothbrush sanitizer.
MedLine Citation:
PMID:  19024257     Owner:  NLM     Status:  MEDLINE    
PURPOSE: This two armed, self-controlled, investigator blinded, clinical study tested the efficacy of an ultraviolet (UV) light toothbrush holder (Violight) to decrease toothbrush bacterial contamination. METHODS: 25 subjects were randomly assigned to control or experimental groups and received two toothbrushes for home use on either even or odd days. The control group rinsed both toothbrushes after use in cold tap water with no mechanical manipulation. The experimental group rinsed one toothbrush in cold running water while storing the other toothbrush in the Violight toothbrush holder after use. The toothbrushes were returned after 2 weeks use in sealed plastic bags and were analyzed for the number of colony forming units (CFU) of S. mutans, S. salivarius, lactobacilli, E. coli, and other coliforms, and total bacterial counts by culture. An additional analysis of the total bacterial profile was performed using denaturing gradient gel electrophoresis (DGGE). RESULTS: The Violight toothbrush holder reduced total CFU by an average of 86% (ANCOVA, P = 0.037). In addition, a tendency was noted for a reduction in total bacterial population as detected by DGGE.
Robert Boylan; Yihong Li; Lidia Simeonova; Gene Sherwin; Judith Kreismann; Ronald G Craig; Jonathan A Ship; Jane A McCutcheon
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of dentistry     Volume:  21     ISSN:  0894-8275     ISO Abbreviation:  Am J Dent     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806701     Medline TA:  Am J Dent     Country:  United States    
Other Details:
Languages:  eng     Pagination:  313-7     Citation Subset:  D    
Department of Basic Sciences and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, New York, New York 10010-4086, USA.
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MeSH Terms
Bacteria / radiation effects*
Colony Count, Microbial
DNA, Bacterial / analysis
Decontamination / instrumentation*
Dental Devices, Home Care / microbiology*
Escherichia coli / radiation effects
Lactobacillus / radiation effects
Middle Aged
Single-Blind Method
Streptococcus / radiation effects
Toothbrushing / instrumentation*
Ultraviolet Rays*
Young Adult
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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