Document Detail

Reduction of dihydroxyphenylacetic acid by a novel enzyme in the rat brain.
MedLine Citation:
PMID:  7503780     Owner:  NLM     Status:  MEDLINE    
A novel enzyme that converts dihydroxyphenylacetic acid (DOPAC) to dihydroxyphenylethanol (DOPET) was found to be present in the microdialysate of the rat brain. The enzyme, named DOPAC reductase, was inhibited by EDTA and stimulated by divalent cations like Zn2+, Mn2+, Co2+ and Cu2+. Its Km, pH optimum and temperature optimum were found to be 32 +/- 2 microM, 7.5 and 40 degrees, respectively. The equivalent acid metabolite of noradrenaline, 3,4-dihydroxymandelic acid, and the methoxylated acids of both noradrenaline and dopamine, 3-methoxy-4-hydroxymandelic acid and 3-methoxy-4-hydroxyphenylacetic acid, were found not to be substrates of DOPAC reductase. Thus, DOPAC reductase may be an enzyme that is specifically involved in the one-step conversion of DOPAC to DOPET in the central metabolism of dopamine.
C L Xu; M K Sim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  50     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-01-18     Completed Date:  1996-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1333-7     Citation Subset:  IM    
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / metabolism*
Brain / enzymology*
Edetic Acid / pharmacology
Enzyme Inhibitors / pharmacology
Mass Spectrometry
Oxidoreductases / antagonists & inhibitors,  metabolism*
Phenylethyl Alcohol / analogs & derivatives,  metabolism
Reg. No./Substance:
0/Enzyme Inhibitors; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 10597-60-1/3,4-dihydroxyphenylethanol; 60-00-4/Edetic Acid; 60-12-8/Phenylethyl Alcohol; EC 1.-/Oxidoreductases; EC 1.-/dihydroxyphenylacetic acid reductase

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