Document Detail


Reduction of cis-dichlorodiammineplatinum-induced cell inactivation by benzaldehyde.
MedLine Citation:
PMID:  6541584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The inactivating effect of a combined treatment of human cells (NHIK 3025) in culture with cis-dichlorodiammineplatinum(II) (cis-DDP) and the protein synthesis inhibitor benzaldehyde was tested. Cell inactivation was measured as loss of colony-forming ability following drug treatment. While 3.2 mM benzaldehyde had no effect on the cell survival when given alone, it reduced the effect of 10 microM cis-DDP significantly when the two drugs were added simultaneously. Scheduling experiments indicate that benzaldehyde must be present immediately before addition of, or simultaneously with, cis-DDP for optimal reduction of cell inactivation. Benzoic acid, benzyl alcohol or the protein synthesis inhibitor cycloheximide did not reduce the inactivating effect of cis-DDP. Cells synchronized by mitotic selection were used to determine the variation in the responses throughout the cell cycle. It was found that concomitant 2-hr treatment of synchronized cells with 3.2 mM benzaldehyde and 10 microM cis-DDP at various times during the cell cycle resulted in a consistently greater surviving fraction of cells than 10 microM cis-DDP alone. Benzaldehyde thus reduced the inactivating effect of cis-DDP in all phases of the cell cycle. The effect of benzaldehyde in combination with two alkylating agents, 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and nitrogen mustard (HN2), was also studied. Benzaldehyde was not found to influence the effects on cell survival induced by these drugs.
Authors:
J M Dornish; E O Pettersen; R Oftebro; J E Melvik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cancer & clinical oncology     Volume:  20     ISSN:  0277-5379     ISO Abbreviation:  Eur J Cancer Clin Oncol     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1984-12-05     Completed Date:  1984-12-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112045     Medline TA:  Eur J Cancer Clin Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1287-93     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Benzaldehydes / pharmacology*
Benzoates / pharmacology
Benzoic Acid
Benzyl Alcohol
Benzyl Alcohols / pharmacology
Cell Cycle / drug effects
Cell Line
Cell Survival / drug effects*
Cisplatin / pharmacology*
Cycloheximide / pharmacology
Female
Humans
Uterine Cervical Neoplasms / pathology
Chemical
Reg. No./Substance:
0/Benzaldehydes; 0/Benzoates; 0/Benzyl Alcohols; 100-51-6/Benzyl Alcohol; 100-52-7/benzaldehyde; 15663-27-1/Cisplatin; 65-85-0/Benzoic Acid; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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