Document Detail


Reduction of the cell cycle length by decreasing G1 phase and cell cycle reentry expand neuronal progenitor cells in the subventricular zone of adult rat after stroke.
MedLine Citation:
PMID:  16251885     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A critical determinant of proliferation of progenitor cells is the duration of the cell division cycle. Stroke increases proliferation of progenitor cells in the subventricular zone (SVZ). Using cumulative and single S-phase labeling with 5-bromo-2'-deoxyuridine, we examined cell cycle kinetics of neural progenitor cells in the SVZ after stroke. In nonstroke rats, 20% of the SVZ cell population was proliferating. However, stroke significantly increased dividing cells up to 31% and these cells had a cell cycle length (T(C)) of 15.3 h, significantly (P < 0.05) shorter than the 19 h Tc in nonstroke SVZ cells. Few terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling-positive cells were detected in the SVZ cells of nonstroke and stroke groups, suggesting that the majority of dividing cells in the SVZ do not undergo apoptosis. Cell cycle phase analysis revealed that stroke substantially shortened the length of the G1 phase (9.6 h) compared with the G1 phase of 12.6 h in nonstroke SVZ cells (P < 0.03). This reduction in G1 contributes to stroke-induced reduction of T(C) because no significant changes were detected on the length of S, G2 and M phases between two groups. Furthermore, compared with progenitor cells in nonstroke SVZ (10%), a greater proportion (14%) of progenitor cells in stroke SVZ reentered the cell cycle after mitosis (P < 0.05). These results show that an increase in proliferating progenitor cells in the SVZ contributes to stroke-induced neurogenesis and this increase is regulated by shortening the length of the cell cycle, decreasing the G1 phase and increasing cell cycle reentry.
Authors:
Rui Lan Zhang; Zheng Gang Zhang; Mei Lu; Ying Wang; James J Yang; Michael Chopp
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  26     ISSN:  0271-678X     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-23     Completed Date:  2006-07-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  857-63     Citation Subset:  IM    
Affiliation:
Department of Neurology; Henry Ford Health Sciences Center, Detroit, Michigan 48202, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology
Brain / pathology,  physiopathology
Cell Cycle / physiology
Cell Proliferation*
G1 Phase / physiology*
Immunohistochemistry
In Situ Nick-End Labeling
Male
Nerve Regeneration / physiology
Neurons / cytology,  pathology,  physiology*
Rats
Stem Cells / cytology,  pathology,  physiology*
Stroke / pathology,  physiopathology*
Time Factors
Grant Support
ID/Acronym/Agency:
P01 NS23393/NS/NINDS NIH HHS; P01 NS42345/NS/NINDS NIH HHS; R01HL 6476/HL/NHLBI NIH HHS; R01NS38292/NS/NINDS NIH HHS

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