Document Detail

Reduction of aberrant crypt foci induced in rat colon with azoxymethane or methylnitrosourea by feeding cholic acid.
MedLine Citation:
PMID:  8422645     Owner:  NLM     Status:  MEDLINE    
Recent studies in our laboratory have demonstrated that feeding cholic acid (CHA) to rats treated with a single dose of azoxymethane (AOM) reduces the growth of putative preneoplastic lesions, aberrant crypt foci (ACF), in a dose-dependent manner. This finding was unexpected since CHA has been reported to promote colon cancer in rats receiving multiple treatments of the colon carcinogen, methylnitrosourea (MNU). The main objective of the present investigation was to evaluate the effect of the type of carcinogen and treatment protocol on the induction and growth of ACF in conjunction with CHA treatment. Male Sprague-Dawley rats received 0, 1 or 2 treatments with AOM or MNU and were fed either the AIN-76A or AIN-76A plus 0.2% CHA diet for 4 weeks. The total number and average size of ACF were significantly reduced in CHA-fed animals regardless of the type or number of treatments of carcinogen. The greatest reduction of ACF due to CHA-feeding was seen in the distal colon. The average crypt multiplicity (number of crypts in each ACF) was not altered by diet or carcinogen treatment. Colonic cell proliferation (crypt height and number of mitotic figures) was significantly increased in CHA-fed animals compared to control diet animals. Therefore, feeding CHA for 4 weeks reduced the number and size of ACF in rats induced by 1 or 2 injections of AOM or MNU, despite stimulation of colonic cell proliferation. These findings suggest further investigation is needed to understand the mechanism of promotion by cholic acid and the value of number and growth characteristics of ACF as a biological endpoint in the pathogenesis of colon cancer.
B A Magnuson; R P Bird
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  68     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-02-22     Completed Date:  1993-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  15-23     Citation Subset:  IM    
Department of Foods and Nutrition, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Analysis of Variance
Azoxymethane / antagonists & inhibitors
Body Weight / drug effects
Cell Division / drug effects
Cholic Acid
Cholic Acids / pharmacology*
Colonic Neoplasms / chemically induced,  pathology,  prevention & control*
Intestinal Mucosa / drug effects,  pathology
Precancerous Conditions / chemically induced,  pathology,  prevention & control*
Random Allocation
Rats, Sprague-Dawley
Reg. No./Substance:
0/Cholic Acids; 25843-45-2/Azoxymethane; 684-93-5/Methylnitrosourea; 81-25-4/Cholic Acid

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