| Reduction of UV-induced cell death in the human senescent fibroblasts. | |
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MedLine Citation:
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PMID: 10987139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied mechanisms by which senescent cells acquire resistance to UV-induced cellular insults. Human primary foreskin fibroblast culture was used since it undergoes cellular senescence in vitro after a limited number of passages. Senescence was induced by a brief treatment of the early passage cells with 100 microM of H2O2 for 1 h, and subsequent culture for 3 weeks. Hydrogen peroxide-treated cells showed an enhancement of senescence-associated beta-galactosidase activity. In the senescent cells, DNA fragmentation in response to UV-irradiation was found to decrease significantly compared with that in the young cells. The SAPK/JNK activation by UV irradiation was reduced in both non-treated senescent cells and the hydrogen peroxide-induced senescent cells, suggesting that a reduced DNA fragmentation by UV-irradiation in the senescent cells is closely related to the decreased SAPK/JNK activity. Since a cell cycle inhibitor, p21Waf1, has been implicated in protecting cells against apoptotic cell death, we determined p21Waf1 to assess whether its elevation has any impact on the reduction of UV-induced activation of SAPK/JNK in the senescent cells. The expression of p21Waf1 increased in both the nontreated and the hydrogen peroxide-treated senescent cells. Our study also revealed that the blockage of SAPK/JNK activation in the senescent cells was closely related to the increased level of p21Waf1. Our observation might provide clues about molecular mechanism of resistance to DNA fragmentation and the consequent cell death by UV-irradiation. |
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Authors:
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E J Yeo; Y C Hwang; C M Kang; H E Choy; S C Park |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecules and cells Volume: 10 ISSN: 1016-8478 ISO Abbreviation: Mol. Cells Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-12-12 Completed Date: 2001-01-18 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9610936 Medline TA: Mol Cells Country: KOREA (SOUTH) |
Other Details:
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Languages: eng Pagination: 415-22 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Seoul National University College of Medicine, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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radiation effects* Cell Culture Techniques Cells, Cultured Child Cyclin-Dependent Kinase Inhibitor p21 Cyclins / metabolism DNA Fragmentation / radiation effects* Fibroblasts / drug effects, metabolism, radiation effects* Humans Hydrogen Peroxide / pharmacology JNK Mitogen-Activated Protein Kinases Male Mitogen-Activated Protein Kinases / metabolism, physiology Radiation Tolerance* Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 7722-84-1/Hydrogen Peroxide; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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