Document Detail

Reduction of Natural Killer and Natural Killer T Cells is Not Protective in Cisplatin-induced Acute Renal Failure in Mice.
MedLine Citation:
PMID:  21557788     Owner:  NLM     Status:  Publisher    
Background/Aims:  A recent report showed that fractalkine (CX3CL1) which functions as both a potent chemoattractant and adhesion molecule for monocytes and natural killer (NK) cells was significantly increased in cisplatin-induced acute renal failure (CisARF) in mice. Therefore, we developed the hypothesis that increased CX3CL1 expression in CisARF initiates NK cell infiltration in the kidney. The aim of the present study was to determine the role of NK cells in CisARF in mice. Methods:  Time course of pan-NK positive cells in CisARF was investigated by using immunohistochemistry (IHC) for CD49b. Pan-NK positive cells were reduced by using anti-NK1.1 mAb. The model of pan-NK positive cells reduction was confirmed by flow cytometry of the spleen and IHC of the kidney. The expression of granzyme A and caspase-1 was examined, and the activity of caspase-1 was also determined. We performed a study on whether there was significant protection of renal function after reduction of pan-NK positive cells. Results:  1) Infiltration of pan-NK positive cells was prominent on day 3 after cisplatin administration. 2) granzyme A expression was significantly increased in CisARF and CisARF+NK1.1 Ab compared to vehicle. 3) Caspase-1 expression and activity was significantly increased in CisARF mice compared to vehicle and CisARF+NK1.1 Ab. 5) Reduction of pan-NK positive cells was not protective in cisplatin-induced acute renal failure in mice. Conclusions:  Although infiltration of pan-NK cells was significantly increased in CisARF, reduction of infiltration of pan-NK cells into the kidney was not protective against CisARF in mice.
Hye-Ryoun Kim; Mi-Kyung Lee; Ae-Ja Park; Eon-Seob Park; Dong-Seok Kim; Jihyun Ahn; Jaetack Kim; Su-Hyun Kim; Dong-Jin Oh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-9
Journal Detail:
Title:  Nephrology (Carlton, Vic.)     Volume:  -     ISSN:  1440-1797     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9615568     Medline TA:  Nephrology (Carlton)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 The Authors. Nephrology © 2011 Asian Pacific Society of Nephrology.
Department of Laboratory Medicine, Pathology, Biochemistry, Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
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