Document Detail

Reducing the glucose uptake rate in Escherichia coli affects growth rate but not protein production.
MedLine Citation:
PMID:  15759256     Owner:  NLM     Status:  MEDLINE    
Although glucose is an inexpensive substrate widely used as a carbon source in Escherichia coli recombinant fermentation technology, 10-30% of the carbon supply is wasted by excreting acetate. In addition to the loss of carbon source, the excretion of a weak acid may result in increased energetic demands and hence a decreased yield. Because glucose can enter the cell via several transport systems, isogenic strains defective in one or two of these transport systems were constructed. The effects of changes in the glucose uptake capacity on the in vivo flux distribution to a desired end product (beta-galactosidase) and to acetate were studied. The lack of one of the components (IICB(Glc) protein) of the glucose-phosphoenolpyruvate phosphotransferase system (Glc-PTS) reduced the growth rate significantly. The maintenance of a low-copy plasmid in this strain resulted in further arrest of the growth rate. However, beta-galactosidase production had no effect on growth rate. This strain directed more carbon into biomass and carbon dioxide, and less into acetate. Beta-galactosidase was produced in amounts not significantly different from the wild-type strain from half the amount of glucose. An explanation for the experimental results is given, making use of published results on metabolic regulation.
A Picon; M J Teixeira de Mattos; P W Postma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  90     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-23     Completed Date:  2005-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  191-200     Citation Subset:  IM    
Copyright Information:
Copyright 2005 Wiley Periodicals, Inc.
Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam, Plantage Muidergracht 12, 1018TV Amsterdam, The Netherlands.
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MeSH Terms
Culture Media
Electrophoresis, Gel, Two-Dimensional
Escherichia coli / growth & development,  metabolism*
Glucose / metabolism*
Recombinant Proteins / biosynthesis*
beta-Galactosidase / biosynthesis
Reg. No./Substance:
0/Culture Media; 0/Recombinant Proteins; 50-99-7/Glucose; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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