Document Detail


Reduced tumorigenicity of a spontaneous mouse lung carcinoma following H-2 gene transfection.
MedLine Citation:
PMID:  3496596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultured cells of the murine lung carcinoma called line 1 express very low levels of H-2 class I antigens and are resistant to lysis mediated by alloreactive T cells. In order to investigate how the expression of class I antigens affects the in vivo growth of this spontaneous tumor, H-2Dp genes were transferred into line 1 cells. Cloned transfectants that displayed H-2Dp surface antigens were identified using flow cytometry. The transfected H-2Dp antigens appeared normal by two-dimensional gel electrophoresis and could also function as excellent targets for T-cell-mediated lysis in vitro. Marked differences in tumorigenicity (defined as tumor growth in immunologically competent hosts) were observed between the Dp transfected cells and untransfected or control transfected line 1 cells in syngeneic mice only if the animals had previously received injections of irradiated Dp transfectants. Expression of Dp antigens did not appreciably affect the growth of line 1 tumors in immunologically naive syngeneic mice or necessarily cause rejection in allogeneic mice. Our in vivo results show that increased expression of class I antigens can reduce the growth of tumors like line 1 that lack all class I antigens. Our results also suggest that increasing class I antigens alone on some spontaneous tumors deficient in expression will not by itself be sufficient for tumor rejection.
Authors:
D W Bahler; J G Frelinger; L W Harwell; E M Lord
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  84     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-08-07     Completed Date:  1987-08-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4562-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Neoplasm / genetics,  physiology*
Carcinoma / genetics,  immunology,  pathology*
Graft Rejection
H-2 Antigens / genetics,  physiology*
Lung Neoplasms / genetics,  immunology,  pathology*
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Neoplasm Transplantation
T-Lymphocytes / immunology
Transfection
Transplantation, Homologous
Grant Support
ID/Acronym/Agency:
5 T32 GM07356/GM/NIGMS NIH HHS; CA 28332/CA/NCI NIH HHS; CA 41448/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/H-2 Antigens; 0/histocompatibility antigen H-2D(b)
Comments/Corrections

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