Document Detail

Reduced swelling-activated Cl(-) current densities in hypertrophied ventricular myocytes of rabbits with heart failure.
MedLine Citation:
PMID:  11922897     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Hypertrophied myocytes of failing hearts have prolonged action potential durations. It is unknown how the swelling-activated Cl(-) current (I(Cl,swell)) affects the abnormal AP configuration. METHODS: We studied I(Cl,swell) in ventricular myocytes isolated from failing and age-matched normal rabbit hearts. We applied whole-cell patch-clamp methodology and activated I(Cl,swell) by lowering tonicity of the superfusate. RESULTS: Neither with ruptured-patch nor with amphotericin B perforated-patch, whole-cell clamp we found I(Cl,swell) active under isotonic conditions in either the normal or the hypertrophied failing heart (HFH) myocytes. I(Cl,swell) caused AP shortening and resting membrane potential (V(m)) depolarization in an osmotic gradient-dependent fashion. However, in the HFH myocytes swelling-induced AP changes were significantly smaller, even though the cells underwent the same relative change in planar cell surface area. Voltage-clamp experiments revealed that in HFH myocytes I(Cl,swell) current density was approximately 50% reduced. CONCLUSION: Reduced I(Cl,swell) densities in HFH myocytes cause limited AP shortening and V(m) depolarization upon swelling of the cells.
Marcel M G J van Borren; Arie O Verkerk; Sakari K Vanharanta; Antonius Baartscheer; Ruben Coronel; Jan H Ravesloot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cardiovascular research     Volume:  53     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-29     Completed Date:  2002-05-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  869-78     Citation Subset:  IM    
Department of Physiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
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MeSH Terms
Action Potentials
Cell Size
Chloride Channels / metabolism*
Heart Failure / metabolism*,  pathology,  physiopathology
Heart Ventricles / cytology,  metabolism
Isotonic Solutions / pharmacology
Membrane Potentials
Osmolar Concentration
Patch-Clamp Techniques
Reg. No./Substance:
0/Chloride Channels; 0/Isotonic Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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