Document Detail


Reduced satellite cell population may lead to contractures in children with cerebral palsy.
MedLine Citation:
PMID:  23210987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: Satellite cells are the stem cells residing in muscle responsible for skeletal muscle growth and repair. Skeletal muscle in cerebral palsy (CP) has impaired longitudinal growth that results in muscle contractures. We hypothesized that the satellite cell population would be reduced in contractured muscle.
METHOD: We compared the satellite cell populations in hamstring muscles from participants with CP contracture (n=8; six males, two females; age range 6-15y; Gross Motor Function Classification System [GMFCS] levels II-V; 4 with hemiplegia, 4 with diplegia) and from typically developing participants (n=8; six males, two females, age range 15-18y). Muscle biopsies were extracted from the gracilis and semitendinosus muscles and mononuclear cells were isolated. Cell surface markers were stained with fluorescently conjugated antibodies to label satellite cells (neural cell adhesion molecule) and inflammatory and endothelial cells (CD34 and CD4 respectively). Cells were analyzed using flow cytometry to determine cell populations.
RESULTS: After gating for intact cells a mean of 12.8% (SD 2.8%) were determined to be satellite cells in typically developing children, but only 5.3% (SD 2.3%; p<0.05) in children with CP. Hematopoietic and endothelial cell types were equivalent in typically developing children and children with CP (p>0.05) suggesting the isolation procedure was valid.
INTERPRETATION: A reduced satellite cell population may account for the decreased longitudinal growth of muscles in CP that develop into fixed contractures or the decreased ability to strengthen muscle in CP. This suggests a unique musculoskeletal disease mechanism and provides a potential therapeutic target for debilitating muscle contractures.
Authors:
Lucas R Smith; Henry G Chambers; Richard L Lieber
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-12-05
Journal Detail:
Title:  Developmental medicine and child neurology     Volume:  55     ISSN:  1469-8749     ISO Abbreviation:  Dev Med Child Neurol     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-12     Completed Date:  2013-04-05     Revised Date:  2014-04-15    
Medline Journal Info:
Nlm Unique ID:  0006761     Medline TA:  Dev Med Child Neurol     Country:  England    
Other Details:
Languages:  eng     Pagination:  264-70     Citation Subset:  IM    
Copyright Information:
© The Authors. Developmental Medicine & Child Neurology © 2012 Mac Keith Press.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Cerebral Palsy / complications*,  pathology*,  physiopathology
Child
Contracture / etiology*,  pathology*,  physiopathology
Female
Flow Cytometry
Humans
Male
Muscle, Skeletal / pathology*,  physiopathology
Satellite Cells, Perineuronal / pathology*
Severity of Illness Index
Grant Support
ID/Acronym/Agency:
AR057393/AR/NIAMS NIH HHS; P30 AR061303/AR/NIAMS NIH HHS; P30AR061303/AR/NIAMS NIH HHS; R01 AR057393/AR/NIAMS NIH HHS; R24 HD050837/HD/NICHD NIH HHS; R24HD050837/HD/NICHD NIH HHS
Comments/Corrections
Comment In:
Dev Med Child Neurol. 2013 Mar;55(3):202   [PMID:  23294400 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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