Document Detail


Reduced protein kinase C activity in a ras-resistant cell line derived from Ki-MSV transformed cells.
MedLine Citation:
PMID:  2830575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined phosphatidylinositol turnover and C-kinase distribution in a flat cellular ras-resistant cell line (C11) derived from Kirsten murine sarcoma virus (Ki-MSV) transformed NIH/3T3 cells (DT). This cell type has been shown to express high levels of the p21 Ki-ras gene product yet is resistant to the transforming effects of this protein. Our data indicate that C11 cells have reduced levels of total C-kinase activity when compared to NIH/3T3 cells and do not retain the ability to phosphorylate the growth associated 80-kDa C-kinase substrate either in vivo or in vitro. Furthermore, whilst the steady state levels of diacylglycerol and the sum of inositol phosphates are elevated in DT cells, in C11 cells these levels are reduced to an amount equivalent to that seen in NIH/3T3 cells. These data indicate a correlation between a protein kinase C dependent pathway and resistance to transformation by ras.
Authors:
T Kamata; N F Sullivan; M W Wooten
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  1     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1988-03-30     Completed Date:  1988-03-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  37-46     Citation Subset:  IM    
Affiliation:
Cold Spring Harbor Laboratory, New York 11724.
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MeSH Terms
Descriptor/Qualifier:
Cell Compartmentation
Cell Line
Cell Membrane / enzymology
Cell Transformation, Viral*
Cytoplasm / enzymology
Diglycerides / metabolism
Gene Expression Regulation / drug effects
Genes, ras*
Kirsten murine sarcoma virus / genetics
Molecular Weight
Phosphatidylinositols / metabolism
Phosphoproteins / metabolism
Protein Kinase C / metabolism*
Tetradecanoylphorbol Acetate / pharmacology
Grant Support
ID/Acronym/Agency:
CA13106/CA/NCI NIH HHS; CA39811/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Diglycerides; 0/Phosphatidylinositols; 0/Phosphoproteins; 16561-29-8/Tetradecanoylphorbol Acetate; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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