Document Detail


Reduced proliferation in the adult mouse subventricular zone increases survival of olfactory bulb interneurons.
MedLine Citation:
PMID:  22363671     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neurogenesis in the adult brain is largely restricted to the subependymal zone (SVZ) of the lateral ventricle, olfactory bulb (OB) and the dentate subgranular zone, and survival of adult-born cells in the OB is influenced by factors including sensory experience. We examined, in mice, whether survival of adult-born cells is also regulated by the rate of precursor proliferation in the SVZ. Precursor proliferation was decreased by depleting the SVZ of dopamine after lesioning dopamine neurons in the substantia nigra compacta with 6-hydroxydopamine. Subsequently, we examined the effect of reduced SVZ proliferation on the generation, migration and survival of neuroblasts and mature adult-born cells in the SVZ, rostral migratory stream (RMS) and OB. Proliferating cells in the SVZ, measured by 5-bromo-2-deoxyuridine (BrdU) injected 2 hours prior to death or by immunoreactivity against Ki67, were reduced by 47% or 36%, respectively, 7 days after dopamine depletion, and by 29% or 31% 42 days after dopamine depletion, compared to sham-treated animals. Neuroblast generation in the SVZ and their migration along the RMS were not affected, neither 7 nor 42 days after the 6-hydroxydopamine injection, since the number of doublecortin-immunoreactive neuroblasts in the SVZ and RMS, as well as the number of neuronal nuclei-immunoreactive cells in the OB, were stable compared to control. However, survival analysis 15 days after 6-hydroxydopamine and 6 days after BrdU injections showed that the number of BrdU+ cells in the SVZ was 70% higher. Also, 42 days after 6-hydroxydopamine and 30 days after BrdU injections, we found an 82% increase in co-labeled BrdU+/γ-aminobutyric acid-immunoreactive cell bodies in the granular cell layer, while double-labeled BrdU+/tyrosine hydroxylase-immunoreactive cell bodies in the glomerular layer increased by 148%. We conclude that the number of OB interneurons following reduced SVZ proliferation is maintained through an increased survival of adult-born precursor cells, neuroblasts and interneurons.
Authors:
Yi Sui; Malcolm K Horne; Davor Stanić
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-21
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-02-24     Completed Date:  2012-06-29     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e31549     Citation Subset:  IM    
Affiliation:
Neurodegeneration Division, Florey Neuroscience Institutes, University of Melbourne, Parkville, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Aging / physiology*
Animals
Astrocytes / cytology,  drug effects,  metabolism
Bromodeoxyuridine / metabolism
Cell Count
Cell Movement / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Dopamine / pharmacology
Dopaminergic Neurons / cytology,  drug effects
Glial Fibrillary Acidic Protein / metabolism
Interneurons / cytology*,  drug effects,  metabolism
Lateral Ventricles / cytology*
Mice
Mice, Inbred C57BL
Microtubule-Associated Proteins / metabolism
Neostriatum / cytology,  drug effects
Neurogenesis / drug effects
Neuropeptides / metabolism
Olfactory Bulb / cytology*
Oxidopamine / pharmacology
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Microtubule-Associated Proteins; 0/Neuropeptides; 0/doublecortin protein; 1199-18-4/Oxidopamine; 59-14-3/Bromodeoxyuridine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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