| Reduced oxygen concentration improves the developmental competence of mouse oocytes following in vitro maturation. | |
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MedLine Citation:
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PMID: 17192892 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reduced atmospheric oxygen concentration is beneficial to embryo development; however, optimal oxygen concentration for oocyte maturation remains undetermined. Likewise, there is no consensus of appropriate medium supplementation during maturation. The objective of this study was to determine whether oxygen tension (20% or 5% O2) and epidermal growth factor (EGF) affect oocyte metabolism and subsequent embryo development. Cumulus-oocyte complexes (COCs) were collected from 28-day-old equine chorionic gonadotropin (eCG) primed or unprimed F1 (C57BL/6xCBA) mice. COCs were matured in defined medium in one of four groups: 20% O2, 20% O2 + EGF, 5% O2, 5% O2 + EGF. In vivo matured COCs were also collected for analysis. COCs from unprimed mice, matured in 5% O2 +/- EGF or 20% O2 + EGF had higher metabolic rates than COCs matured in 20% O2 (P < 0.05). COCs from primed mice had higher metabolic rates when matured in the presence of EGF, regardless of oxygen tension (P < 0.01). Oxygen uptake and mitochondrial membrane potential were higher for in vivo matured oocytes and oocytes matured under 5% O2 compared to oocytes matured under 20% O2 (P < 0.05). Blastocyst formation was not different between maturation groups (primed or unprimed); however, embryo cell numbers were 20-45% significantly higher when COCs were matured at 5% O2 (P < 0.05). Results suggest that oocytes matured in physiological concentrations of oxygen have improved development and metabolic activity, more closely resembling in vivo maturation. These findings have implications for oocyte maturation in both clinical and research laboratories. |
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Authors:
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Kimberly A Preis; George E Seidel; David K Gardner |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular reproduction and development Volume: 74 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-05-01 Completed Date: 2007-08-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: United States |
Other Details:
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Languages: eng Pagination: 893-903 Citation Subset: IM |
Affiliation:
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Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chorionic Gonadotropin / metabolism Epidermal Growth Factor / metabolism Female Glucose / metabolism Humans Membrane Potentials Mice Mice, Inbred C57BL Mitochondria / metabolism Oocytes / cytology, metabolism*, physiology* Oxygen / metabolism* Oxygen Consumption Phloretin / metabolism Phlorhizin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 50-99-7/Glucose; 60-81-1/Phlorhizin; 60-82-2/Phloretin; 62229-50-9/Epidermal Growth Factor; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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