Document Detail


Reduced ocular blood flow as an early indicator of diabetic retinopathy in a mouse model of diabetes.
MedLine Citation:
PMID:  22915034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To investigate ocular blood flow and visual function in the Ins2(Akita) diabetic retinopathy mouse model at early and late time points after onset of hyperglycemia.
METHODS: Mice heterozygous for the Ins2(Akita) mutation, which become hyperglycemic at approximately 4 weeks old, were studied at 2.5 and 7.5 months of age, with age-matched wild-type littermates used as controls. Retinal and choroidal blood flows were noninvasively imaged at 42 × 42 × 400 μm using magnetic resonance imaging. Visual function was measured using optokinetic tracking to determine spatial frequency and contrast thresholds from the same mice.
RESULTS: At 2.5 months, choroidal blood flow was significantly reduced (P < 0.01) by 20% in Ins2(Akita) mice (n = 13) compared with age-matched controls (n = 16), whereas retinal blood flow and visual function were not significantly affected (P > 0.05). At 7.5 months, both choroidal and retinal blood flow were significantly reduced (P < 0.05) by 27% and 28%, respectively, in Ins2(Akita) mice (n = 11) compared with age-matched controls (n = 15). Visual functions were also significantly worse (P < 0.05) in Ins2(Akita) mice at 7.5 months, as indicated by a 19% decreased spatial frequency threshold and 135% increased contrast threshold compared with age-matched controls. The magnitudes of the blood flow and vision deficits, however, were not correlated.
CONCLUSIONS: Although both choroidal and retinal blood flow and vision were altered after prolonged diabetes in the Ins2(Akita) mouse, choroidal blood flow was reduced even in young diabetic animals, suggesting ocular blood flow deficit could be an early pathological change in diabetic retinopathy.
Authors:
Eric R Muir; René C Rentería; Timothy Q Duong
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-09-21
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  53     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-24     Completed Date:  2012-12-10     Revised Date:  2014-03-31    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6488-94     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Choroid / blood supply,  physiology
Contrast Sensitivity / physiology
Diabetic Retinopathy / genetics,  pathology*,  physiopathology*
Disease Models, Animal
Early Diagnosis
Female
Hyperglycemia / genetics,  physiopathology
Insulin / genetics
Male
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Photic Stimulation / methods
Regional Blood Flow / physiology*
Retina / pathology,  physiopathology
Retinal Vessels / pathology,  physiopathology*
Sensory Thresholds / physiology
Grant Support
ID/Acronym/Agency:
5T32HL007446-29/HL/NHLBI NIH HHS; 8UL1TR000149/TR/NCATS NIH HHS; EY018855/EY/NEI NIH HHS; R01 EY014211/EY/NEI NIH HHS; R01 EY014211/EY/NEI NIH HHS; R01 EY018855/EY/NEI NIH HHS; T32 HL007446/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Ins2 protein, mouse; 0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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