Document Detail


Reduced levels of recent thymic emigrants in acute myeloid leukemia patients.
MedLine Citation:
PMID:  19018534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: T cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Our previous study showed skewed expression of T cell receptor beta variable region (TRBV) subfamilies and clonal expansion of T cells in leukemia patients. In the present study, in order to further characterize the T cell immunity in acute myeloid leukemia (AML) patients, the level of recent thymic emigrants (RTE) was analyzed. MATERIALS AND METHODS: Quantitative analysis of signal joint T cell recombination excision circles (deltaRec-psiJalpha sjTRECs) was performed in peripheral blood mononuclear cells (PBMCs) by real-time PCR (TaqMan), and the analysis of 23 TRBV-BD1 sjTRECs was performed by semi-nested PCR. Eighty-eight cases with AML were selected for this study; ten AML cases in complete remission (AML-CR) and 38 healthy individuals served as controls. RESULTS: The levels of deltaRec-psiJalpha sjTRECs in PBMCs and CD3+ T cells were significantly decreased in AML patients, compared with healthy individuals and in patients in completive remission. Also the frequency of 23 TRBV-BD1 sjTRECs, and the number of detectable TRBV subfamily sjTRECs were significantly lower in AML patients than in healthy individuals. Moreover, the sjTRECs numbers and the frequency of TRBV-BD1 sjTRECs showed a progressive linear decline with age in AML patients. CONCLUSIONS: The decreased numbers of universal (deltaRec-psiJalpha) and family-specific (TRBV-BD1) sjTRECs indicate that the severe T cell immunodeficiency in AML patients is associated with reduced levels of recent thymic emigrants. In patients achieving complete remission both sjTREC counts return to normal values indicating the recovery of thymic function. Better understanding of the mechanisms underlying persistent immunodeficiency in leukemia patients may lead to novel treatment strategies to enhance immune competence.
Authors:
Yangqiu Li; Qingsong Yin; Lijian Yang; Shaohua Chen; Suxia Geng; Xiuli Wu; Liye Zhong; Christian A Schmidt; Grzegorz K Przybylski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-19
Journal Detail:
Title:  Cancer immunology, immunotherapy : CII     Volume:  58     ISSN:  1432-0851     ISO Abbreviation:  Cancer Immunol. Immunother.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-04-28     Completed Date:  2009-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605732     Medline TA:  Cancer Immunol Immunother     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1047-55     Citation Subset:  IM    
Affiliation:
Institute of Hematology, Medical College, Jinan University, 510632 Guangzhou, China. yangqiuli@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Cell Movement* / genetics
Child
Child, Preschool
Female
Gene Frequency
Humans
Leukemia, Myeloid, Acute / immunology*
Male
Middle Aged
Receptors, Antigen, T-Cell, alpha-beta / genetics,  immunology*
T-Lymphocytes / immunology*
Thymus Gland / immunology*
Young Adult
Chemical
Reg. No./Substance:
0/Receptors, Antigen, T-Cell, alpha-beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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