Document Detail

Reduced levels of cathepsin D associated with tamoxifen resistance and estrogen independence in the ZR-75-1 human breast cancer cell line.
MedLine Citation:
PMID:  8616829     Owner:  NLM     Status:  MEDLINE    
The expression and secretion of cathepsin D by ZR-75-1 human breast cancer cells, and tamoxifen-resistant (ZR-75-9a1) and estrogen-independent (ZR-PR-LT) variants was examined by electrophoresis of labeled proteins and Western blotting. Secreted proteins of 160 kDa, 52 kDa and 34 kDa were identified, and in ZR-75-1 cells, they were shown to be estrogen-inducible. Treatment of ZR-75-9a1 cells with 17 beta-estradiol (E2) and the progestin ORG 2058 increased secretion of the 52 kDa protein; ZR-PR-LT cells were unaffected. Western blotting showed that each cell line expressed high levels of the 52 kDa and 34 kDa forms of cathepsin D but that relatively little was being secreted. Each cell line secreted 52 kDa procathepsin D, but 34 kDa mature-cathepsin D was not detected as a secreted protein. ZR-75-1 cells expressed and secreted the highest levels of cathepsin D while ZR-75-9a1 cells expressed and secreted the least.
B J Long; H W van den Berg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  99     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-06-13     Completed Date:  1996-06-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  233-8     Citation Subset:  IM    
Division of Nutrition and Endocrinology, American Health Foundation, Valhalla, NY 10595, USA.
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MeSH Terms
Breast Neoplasms
Cathepsin D / biosynthesis,  metabolism*
Cell Line
Clone Cells
Drug Resistance, Neoplasm*
Estradiol / pharmacology*
Gene Expression / drug effects
Molecular Weight
Pregnenediones / pharmacology*
Progesterone Congeners / pharmacology*
Receptor, Epidermal Growth Factor / analysis
Receptors, Estrogen / analysis
Receptors, Progesterone / analysis
Tamoxifen / toxicity*
Tumor Cells, Cultured
Reg. No./Substance:
0/Pregnenediones; 0/Progesterone Congeners; 0/Receptors, Estrogen; 0/Receptors, Progesterone; 10540-29-1/Tamoxifen; 24320-06-7/16 alpha-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione; 50-28-2/Estradiol; EC, Epidermal Growth Factor; EC D

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