| Reduced inflammatory potential of peritoneal macrophages recruited in mice pretreated with a glycolipid synthesis inhibitor. | |
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MedLine Citation:
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PMID: 16983496 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Integral components of mammalian cell membranes, glycosphingolipids (GSL) reside in specialized plasma membrane microdomains critical for cell signaling. N-alkylated nojirimycins are compounds developed for GSL substrate deprivation therapy, blocking GSL synthesis by specifically inhibiting an essential enzyme, ceramide glucosyltransferase. Peritoneal macrophages recruited in mice pretreated with an inhibitory N-alkylnojirimycin displayed a reduced capacity to release either TNFalpha or interleukin-6 when re-exposed to whole killed E. coli in vitro. Cell viability and protein content were not affected. A nojirimycin analogue without GSL inhibitory capacity had no effect. The results show inhibition of GSL synthesis in vivo by an N-alkylnojirimycin can reduce the response to an inflammatory stimulus and indicate N-alkylnojirimycins have experimental and potential clinical value for modulating innate immune responses in vivo. |
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Authors:
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H C Yohe; T J Kitzmiller; W J Bement; B E Dwyer; J F Sinclair |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Inflammopharmacology Volume: 14 ISSN: 0925-4692 ISO Abbreviation: Inflammopharmacology Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-09-19 Completed Date: 2006-10-13 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9112626 Medline TA: Inflammopharmacology Country: Switzerland |
Other Details:
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Languages: eng Pagination: 170-5 Citation Subset: IM |
Affiliation:
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Research Service, Department of Veterans Affairs, VA RO & MC, 215 North Main Street, White River Junction, VT 05009, USA. herbert.c.yohe@dartmouth.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Deoxynojirimycin
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analogs & derivatives Animals Cell Survival / drug effects Cytokines / metabolism Enzyme Inhibitors / pharmacology* Glucosamine / analogs & derivatives*, pharmacology Glucosyltransferases / antagonists & inhibitors* Glycosphingolipids / antagonists & inhibitors*, biosynthesis Immunity, Innate / drug effects* Macrophages, Peritoneal* / drug effects, enzymology, immunology, metabolism Mice Mice, Inbred C3H |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Enzyme Inhibitors; 0/Glycosphingolipids; 15218-38-9/nojirimycin; 19130-96-2/1-Deoxynojirimycin; 3416-24-8/Glucosamine; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.80/ceramide glucosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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