Document Detail

Reduced induction of HSP70 in PC12 cells during neuronal differentiation.
MedLine Citation:
PMID:  9443804     Owner:  NLM     Status:  MEDLINE    
Rat pheochromocytoma PC12 cells differentiate into nonreplicating neuronal cells with neurite extensions in response to nerve growth factor (NGF). To gain better understanding of the regulation of stress responses in neuronal cells, we examined the induction of HSP70, HSP70 mRNA, and heat shock factor 1 (HSF1) DNA-binding activity following treatment by heat shock or with sodium arsenite or amino acid analogue in PC12 cells treated with or without NGF. The induction of HSP70 and HSP70 mRNA following these stresses was diminished in the differentiated PC12 cells compared to the undifferentiated cells, whereas the HSF1 DNA-binding activity was enhanced in the differentiated PC12 cells. This phenomenon was characteristic of the differentiated neuronal cells rather than growth-arrested cells. Thus, neuronal cells appear to show an altered stress response depending on their differentiation state, and the diminished HSP70 expression in the differentiated neuronal cells may explain the sensitivity of neuronal cells to pathophysiological stressors.
T Hatayama; H Takahashi; N Yamagishi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  122     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-02-17     Completed Date:  1998-02-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  904-10     Citation Subset:  IM    
Department of Biochemistry, Kyoto Pharmaceutical University.
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MeSH Terms
Arsenites / pharmacology
Azetidinecarboxylic Acid / pharmacology
Cell Differentiation / drug effects
Culture Media, Serum-Free
Down-Regulation / drug effects,  genetics
HSP70 Heat-Shock Proteins / biosynthesis*,  genetics
Hot Temperature
Nerve Growth Factors / pharmacology
Neurons / drug effects,  metabolism*,  pathology*
PC12 Cells / drug effects,  metabolism*,  pathology*
RNA, Messenger / biosynthesis
Sodium Compounds / pharmacology
Stress, Physiological / chemically induced,  metabolism,  pathology
Reg. No./Substance:
0/Arsenites; 0/Culture Media, Serum-Free; 0/HSP70 Heat-Shock Proteins; 0/Nerve Growth Factors; 0/RNA, Messenger; 0/Sodium Compounds; 13768-07-5/sodium arsenite; 2517-04-6/Azetidinecarboxylic Acid

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