| Reduced expression of fatty acid biosynthesis genes in the prefrontal cortex of patients with major depressive disorder. | |
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MedLine Citation:
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PMID: 20863572 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Major depressive disorder (MDD) is associated with central and peripheral deficits in long-chain polyunsaturated fatty acids (LC-PUFA), particularly those in the omega-3 fatty acid family. However, the etiology of these deficits remains poorly understood, and there is currently little known about the expression of genes that mediate fatty acid biosynthesis in MDD patients. METHODS: The expression of FADS1 (Δ5 desaturase), FADS2 (Δ6 desaturase), HELO1 [ELOVL5] (elongase), PEX19 (peroxisome), and SCD (stearoyl-CoA desaturase [Δ9 desaturase]) was determined in the postmortem prefrontal cortex of MDD patients (n=10) and non-psychiatric controls (n=10) by real-time reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: After correcting for multiple comparisons, FADS1 mRNA expression was significantly lower in MDD patients relative to controls (-27%, p=0.009), and there were trends for lower expression of FADS2 (-30%, p=0.07), HELO1 (-37%, p=0.02), and SCD (-43%, p=0.02). PEX19 mRNA expression did not differ between controls and MDD patients (-2%, p=0.92). There were no significant gender effects, and relative reductions in FADS1, HELO1, and SCD expression were greater in patients that did not commit suicide compared with patients that did commit suicide. LIMITATIONS: The sample size was small, and all MDD patients were receiving antidepressant medications. CONCLUSIONS: Principal genes involved in LC-PUFA and monounsaturated fatty acid biosynthesis are down-regulated in the postmortem prefrontal cortex of MDD patients. Additional studies are needed to replicate and extend these findings in a larger sample that includes antidepressant-free MDD patients. |
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Authors:
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Robert K McNamara; Yanhong Liu |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-21 |
Journal Detail:
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Title: Journal of affective disorders Volume: 129 ISSN: 1573-2517 ISO Abbreviation: J Affect Disord Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-14 Completed Date: 2011-06-13 Revised Date: 2012-09-24 |
Medline Journal Info:
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Nlm Unique ID: 7906073 Medline TA: J Affect Disord Country: Netherlands |
Other Details:
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Languages: eng Pagination: 359-63 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Psychiatry, Lipidomics Research Program, University of Cincinnati College of Medicine, Cincinnati, OH 45219, United States. robert.mcnamara@uc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetyltransferases
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metabolism Adult Aged Case-Control Studies Depressive Disorder, Major / metabolism* Fatty Acid Desaturases / metabolism Fatty Acids, Unsaturated / biosynthesis*, genetics Female Gene Expression* Humans Male Membrane Proteins / metabolism Middle Aged Prefrontal Cortex / metabolism* Reverse Transcriptase Polymerase Chain Reaction Stearoyl-CoA Desaturase / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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MH073704/MH/NIMH NIH HHS; MH074858/MH/NIMH NIH HHS; R21 MH073704-02/MH/NIMH NIH HHS; R21 MH074858-01A1/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Membrane Proteins; 157153-79-2/PEX19 protein, human; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.19.1/Stearoyl-CoA Desaturase; EC 1.14.19.3/FADS2 protein, human; EC 1.14.99.-/delta-5 fatty acid desaturase; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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