Document Detail

Reduced expression of fatty acid biosynthesis genes in the prefrontal cortex of patients with major depressive disorder.
MedLine Citation:
PMID:  20863572     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Major depressive disorder (MDD) is associated with central and peripheral deficits in long-chain polyunsaturated fatty acids (LC-PUFA), particularly those in the omega-3 fatty acid family. However, the etiology of these deficits remains poorly understood, and there is currently little known about the expression of genes that mediate fatty acid biosynthesis in MDD patients.
METHODS: The expression of FADS1 (Δ5 desaturase), FADS2 (Δ6 desaturase), HELO1 [ELOVL5] (elongase), PEX19 (peroxisome), and SCD (stearoyl-CoA desaturase [Δ9 desaturase]) was determined in the postmortem prefrontal cortex of MDD patients (n=10) and non-psychiatric controls (n=10) by real-time reverse transcriptase polymerase chain reaction (RT-PCR).
RESULTS: After correcting for multiple comparisons, FADS1 mRNA expression was significantly lower in MDD patients relative to controls (-27%, p=0.009), and there were trends for lower expression of FADS2 (-30%, p=0.07), HELO1 (-37%, p=0.02), and SCD (-43%, p=0.02). PEX19 mRNA expression did not differ between controls and MDD patients (-2%, p=0.92). There were no significant gender effects, and relative reductions in FADS1, HELO1, and SCD expression were greater in patients that did not commit suicide compared with patients that did commit suicide.
LIMITATIONS: The sample size was small, and all MDD patients were receiving antidepressant medications.
CONCLUSIONS: Principal genes involved in LC-PUFA and monounsaturated fatty acid biosynthesis are down-regulated in the postmortem prefrontal cortex of MDD patients. Additional studies are needed to replicate and extend these findings in a larger sample that includes antidepressant-free MDD patients.
Robert K McNamara; Yanhong Liu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-09-21
Journal Detail:
Title:  Journal of affective disorders     Volume:  129     ISSN:  1573-2517     ISO Abbreviation:  J Affect Disord     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-14     Completed Date:  2011-06-13     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  7906073     Medline TA:  J Affect Disord     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  359-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Acetyltransferases / metabolism
Case-Control Studies
Depressive Disorder, Major / metabolism*
Fatty Acid Desaturases / metabolism
Fatty Acids, Unsaturated / biosynthesis*,  genetics
Gene Expression*
Membrane Proteins / metabolism
Middle Aged
Prefrontal Cortex / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Stearoyl-CoA Desaturase / metabolism
Grant Support
MH073704/MH/NIMH NIH HHS; MH074858/MH/NIMH NIH HHS; R21 MH073704/MH/NIMH NIH HHS; R21 MH073704-02/MH/NIMH NIH HHS; R21 MH074858/MH/NIMH NIH HHS; R21 MH074858-01A1/MH/NIMH NIH HHS
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Membrane Proteins; 157153-79-2/PEX19 protein, human; EC 1.14.19.-/Fatty Acid Desaturases; EC Desaturase; EC protein, human; EC 1.14.99.-/delta-5 fatty acid desaturase; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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