| Reduced expression of INT-6/eIF3-p48 in human tumors. | |
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MedLine Citation:
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PMID: 11115556 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The int-6 gene, originally identified as a common integration site for the mouse mammary tumor virus (MMTV) in mouse mammary tumors, encodes the p48 component of the eukaryotic translation initiation factor-3 (eIF3-p48). Int-6/eIF3-p48 is expressed in all adult tissues which have been tested and early in embryonic development. Int-6/eIF3-p48 has been highly conserved throughout evolution and the deduced amino acid sequence of the human gene product is identical to the mouse protein. Viral insertions at the Int-6/eIF3-p48 locus in mouse mammary tumors result in production of chimeric Int-6/eIF3-p48/MMTV products that may act as dominant negative oncoproteins. Int-6/eIF3-p48 has also been identified as a human protein that binds to the human T-cell leukemia virus type I Tax oncoprotein. The role of Int-6/eIF3-p48 in human carcinogenesis is unknown at the present time. In this study we have examined Int-6/eIF3-p48 gene status and expression in two of the most common forms of cancer in humans, breast and lung tumors. Sixty-two breast carcinomas and 78 non-small cell lung carcinomas (NSCLC) were investigated. LOH at the Int-6/eIF3-p48 locus was observed in 5 (21%) of 24 informative breast tumors and 10 (29%) of 34 informative lung tumors. A reduced expression of Int-6/eIF3-p48 was seen in 23 (37%) of breast cancer samples and 24 (31%) of NSCLC samples. An association between Int-6/eIF3-p48 expression and LOH at the Int-6/eIF3-p48 locus was observed. Int-6/eIF3-p48 expression was not related to commonly used pathological parameters in breast cancer patients, while in NSCLC patients int-6/eIF3-p48 expression was mainly seen in adenocarcinomas (P<0.0001). In conclusion, our data show for the first time a decreased expression of Int-6/eIF3-p48 in a consistent portion of human breast and lung carcinomas, frequently associated with LOH at the Int-6/eIF3-p48 locus. Additional studies on larger series of tumor specimens with long-term follow-up are needed to determine whether Int-6/eIF3-p48 expression may represent a new prognostic or predictive marker. |
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Authors:
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A Marchetti; F Buttitta; S Pellegrini; G Bertacca; R Callahan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of oncology Volume: 18 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-08 Completed Date: 2001-02-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: GREECE |
Other Details:
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Languages: eng Pagination: 175-9 Citation Subset: IM |
Affiliation:
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Department of Oncology and Neurosciences, University Gabriele D'Annunzio, Chieti, Italy. amarchetti@unich.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms
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diagnosis,
genetics*,
metabolism Eukaryotic Initiation Factor-3 Female Humans Loss of Heterozygosity / genetics Lung Neoplasms / diagnosis, genetics*, metabolism Male Prognosis Proto-Oncogene Proteins / genetics*, metabolism RNA, Messenger / metabolism Tumor Markers, Biological / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Eukaryotic Initiation Factor-3; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/Tumor Markers, Biological |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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