| Reduced expression of C1q-mRNA in monocytes from patients with systemic lupus erythematosus. | |
| | |
MedLine Citation:
|
PMID: 17100759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Inherited C1q deficiency is associated strongly with the development of systemic lupus erythematosus (SLE). The aim of our study was to evaluate the ability of monocytes from SLE patients without inherited C1q deficiency to up-regulate C1q-mRNA upon stimulation. Furthermore, we wanted to elucidate the physiological stimulus for up-regulation of C1q-mRNA. Peripheral blood mononuclear cell (PBMC)-derived monocytes from 10 SLE patients, 10 patients with rheumatoid arthritis (RA) and 10 healthy controls (HC) were stimulated with dexamethasone (DXM), interferon-gamma or both. Additionally, purified monocytes from HC were stimulated with interleukin (IL)-10. C1q-mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction (RT-PCR). C1q protein was detected using the standard alkaline phosphatase/anti-alkaline phosphatase (APAAP) technique. SLE monocytes were significantly less able to up-regulate C1q-mRNA when compared to RA or HC. IL-10 was identified as an important stimulus for C1q synthesis. In SLE patients there is a significant functional impairment of monocytes to synthesize C1q upon stimulation. As C1q is linked to the process of recognition and removal of apoptotic cells, this relative C1q deficiency is likely to contribute to the reduced phagocytosis of apoptotic material observed in SLE and thereby might be a central pathogenetic factor. |
| | |
Authors:
|
F Moosig; F Damm; A Knorr-Spahr; M Ritgen; R A Zeuner; M Kneba; M Ernst; J O Schröder |
Related Documents
:
|
22044749 - Effects of the polysaccharide from pholiota nameko on human cytokine network in serum. 10770289 - Neurotransmitters of the sympathetic nerve terminal are powerful chemoattractants for m... 15047569 - Differential expression of pde4 camp phosphodiesterase isoforms in inflammatory cells o... 1999239 - The human leukemia cell line, thp-1: a multifacetted model for the study of monocyte-ma... 2967429 - Appearance of acceptor-bound c3b on hla-dr positive macrophages and on stimulated u937 ... 1582709 - Influence of dexamethasone on the rm 3/1-positive macrophages in the peripheral blood a... 11292609 - Nf-kappab activation and susceptibility to apoptosis after polyamine depletion in intes... 17606399 - Administration of keratinocyte growth factor down-regulates the pulmonary capacity of a... 9481089 - Cytokines and local cellular immunity. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Clinical and experimental immunology Volume: 146 ISSN: 0009-9104 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2006 Dec |
Date Detail:
|
Created Date: 2006-11-14 Completed Date: 2007-02-20 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
|
Languages: eng Pagination: 409-16 Citation Subset: IM |
Affiliation:
|
University of Schleswig Holstein, Campus Kiel, Kiel, Germany, and Forschungszentrum Borstel, Borstel, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Anti-Inflammatory Agents / pharmacology Arthritis, Rheumatoid / immunology Cells, Cultured Complement C1q / biosynthesis*, genetics Dexamethasone / pharmacology Female Humans Immunoenzyme Techniques Interferon-gamma / immunology Interleukin-10 / immunology Lupus Erythematosus, Systemic / immunology* Middle Aged Monocytes / immunology* RNA, Messenger / genetics Up-Regulation / drug effects, immunology |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Inflammatory Agents; 0/RNA, Messenger; 130068-27-8/Interleukin-10; 50-02-2/Dexamethasone; 80295-33-6/Complement C1q; 82115-62-6/Interferon-gamma |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: CD80/CD28 co-stimulation in human brucellosis.
Next Document: Nickel, cobalt, chromium, palladium and gold induce a mixed Th1- and Th2-type cytokine response in v...