Document Detail

Reduced expression of C1q-mRNA in monocytes from patients with systemic lupus erythematosus.
MedLine Citation:
PMID:  17100759     Owner:  NLM     Status:  MEDLINE    
Inherited C1q deficiency is associated strongly with the development of systemic lupus erythematosus (SLE). The aim of our study was to evaluate the ability of monocytes from SLE patients without inherited C1q deficiency to up-regulate C1q-mRNA upon stimulation. Furthermore, we wanted to elucidate the physiological stimulus for up-regulation of C1q-mRNA. Peripheral blood mononuclear cell (PBMC)-derived monocytes from 10 SLE patients, 10 patients with rheumatoid arthritis (RA) and 10 healthy controls (HC) were stimulated with dexamethasone (DXM), interferon-gamma or both. Additionally, purified monocytes from HC were stimulated with interleukin (IL)-10. C1q-mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction (RT-PCR). C1q protein was detected using the standard alkaline phosphatase/anti-alkaline phosphatase (APAAP) technique. SLE monocytes were significantly less able to up-regulate C1q-mRNA when compared to RA or HC. IL-10 was identified as an important stimulus for C1q synthesis. In SLE patients there is a significant functional impairment of monocytes to synthesize C1q upon stimulation. As C1q is linked to the process of recognition and removal of apoptotic cells, this relative C1q deficiency is likely to contribute to the reduced phagocytosis of apoptotic material observed in SLE and thereby might be a central pathogenetic factor.
F Moosig; F Damm; A Knorr-Spahr; M Ritgen; R A Zeuner; M Kneba; M Ernst; J O Schröder
Related Documents :
22044749 - Effects of the polysaccharide from pholiota nameko on human cytokine network in serum.
10770289 - Neurotransmitters of the sympathetic nerve terminal are powerful chemoattractants for m...
15047569 - Differential expression of pde4 camp phosphodiesterase isoforms in inflammatory cells o...
1999239 - The human leukemia cell line, thp-1: a multifacetted model for the study of monocyte-ma...
2967429 - Appearance of acceptor-bound c3b on hla-dr positive macrophages and on stimulated u937 ...
1582709 - Influence of dexamethasone on the rm 3/1-positive macrophages in the peripheral blood a...
23948289 - Notch 1 signalling inhibits cardiomyocyte apoptosis in ischaemic postconditioning.
11292609 - Nf-kappab activation and susceptibility to apoptosis after polyamine depletion in intes...
17606399 - Administration of keratinocyte growth factor down-regulates the pulmonary capacity of a...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  146     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-14     Completed Date:  2007-02-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  409-16     Citation Subset:  IM    
University of Schleswig Holstein, Campus Kiel, Kiel, Germany, and Forschungszentrum Borstel, Borstel, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anti-Inflammatory Agents / pharmacology
Arthritis, Rheumatoid / immunology
Cells, Cultured
Complement C1q / biosynthesis*,  genetics
Dexamethasone / pharmacology
Immunoenzyme Techniques
Interferon-gamma / immunology
Interleukin-10 / immunology
Lupus Erythematosus, Systemic / immunology*
Middle Aged
Monocytes / immunology*
RNA, Messenger / genetics
Up-Regulation / drug effects,  immunology
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/RNA, Messenger; 130068-27-8/Interleukin-10; 50-02-2/Dexamethasone; 80295-33-6/Complement C1q; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  CD80/CD28 co-stimulation in human brucellosis.
Next Document:  Nickel, cobalt, chromium, palladium and gold induce a mixed Th1- and Th2-type cytokine response in v...