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Reduced contact-inhibition and substratum adhesion in epithelial cells expressing GlcNAc-transferase V.
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MedLine Citation:
PMID:  7615638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Malignant transformation of fibroblast and epithelial cells is accompanied by increased beta 1-6 N-acetylglucosaminyltransferase V (GlcNAc-TV) activity, a Golgi N-linked oligosaccharide processing enzyme. Herein, we report that expression of GlcNAc-TV in Mv1Lu cells, an immortalized lung epithelial cell line results in loss of contact-inhibition of cell growth, an effect that was blocked by swainsonine, an inhibitor of Golgi processing enzyme alpha-mannosidase II. In serum-deprived and high density monolayer cultures, the GlcNAc-TV transfectants formed foci, maintained microfilaments characteristic of proliferating cells, and also experienced accelerated cell death by apoptosis. Injection of the GlcNAc-TV transfectants into nude mice produced a 50% incidence of benign tumors, and progressively growing tumors in 2:12 mice with a latency of 6 mo, while no growth was observed in mice injected with control cells. In short term adhesion assays, the GlcNAc-TV expressing cells were less adhesive on surfaces coated with fibronectin and collagen type IV, but no changes were observed in levels of cell surface alpha 5 beta 1 or alpha v beta 3 integrins. The larger apparent molecular weights of the LAMP-2 glycoprotein and integrin glycoproteins alpha 5, alpha v and beta 1 in the transfected cells indicates that their oligosaccharide chains are substrates for GlcNAc-TV. The results suggest that beta 1-6GlcNAc branching of N-linked oligosaccharides contributes directly to relaxed growth controls and reduce substratum adhesion in premalignant epithelial cells.
Authors:
M Demetriou; I R Nabi; M Coppolino; S Dedhar; J W Dennis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  130     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-08-22     Completed Date:  1995-08-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  383-92     Citation Subset:  IM    
Affiliation:
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD*
Apoptosis
Cell Adhesion*
Cell Line
Cell Movement
Cell Survival
Contact Inhibition*
Epithelial Cells
Lysosome-Associated Membrane Glycoproteins
Membrane Glycoproteins / metabolism
Mice
Mice, Nude
Microfilaments / ultrastructure
Mink
N-Acetylglucosaminyltransferases / metabolism*
Neoplasms, Experimental / etiology
Oligosaccharides / metabolism
Swainsonine / pharmacology
Transfection
Transforming Growth Factor beta / pharmacology
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Lysosome-Associated Membrane Glycoproteins; 0/Membrane Glycoproteins; 0/Oligosaccharides; 0/Transforming Growth Factor beta; 72741-87-8/Swainsonine; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC 2.4.1.155/alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 2 Month: 7 Year: 1995
Volume: 130 Issue: 2
First Page: 383 Last Page: 392
ID: 2199932
Publisher Id: 95340638
PubMed Id: 7615638

Reduced contact-inhibition and substratum adhesion in epithelial cells expressing GlcNAc-transferase V


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