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Reduced apoptosis in term placentas from gestational diabetic pregnancies.
MedLine Citation:
PMID:  25054844     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Gestational diabetic mellitus (GDM) pregnancies have an increased risk of macrosomic infants and large placental mass, though the mechanisms explaining each of these is uncertain. We sought to evaluate the contribution of apoptosis to placental size and the expression of glucose transporters (SLC2A) in GDM pregnancies. Maternal age and pre-pregnancy body weight were documented. Newborn weights were recorded after delivery. Placentas 37-40-week gestation from control patients (no pregnancy complication) (n = 5), or with GDM (n = 5) were weighed immediately after delivery. Villous samples (4 mm diameter) were collected and divided into specimens; one was fixed in 4% paraformaldehyde for immunostaining using terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL) and activated caspase-3. The other specimen was snap frozen in liquid nitrogen and stored at -80°C for active caspase-3, poly(ADP-ribose) polymerase (PARP), SLC2A1 and SLC2A3 gene expression analysis. Our results showed that maternal age and pre-pregnancy body weight were significantly higher in the GDM group when compared with those from the controls (P < 0.05). The mean neonatal birth weight and placenta weight were significantly higher in the GDM group compared with that from the controls (P < 0.05). The apoptotic index of placentas (0.05 ± 0.01 v. 0.17 ± 0.04, P < 0.04), active caspase-3 polypeptide fragments and PARP protein were significantly decreased in GDM placentas as compared with controls. Further, the level of placental SLC2A1 protein expression was ∼3-fold higher in GDM placentas. Our results suggest that reduced apoptosis in GDM placentas may contribute to increased placental tissue, which together with enhanced SLC2A1 expression, could play a role in fetal macrosomia.
Authors:
L Belkacemi; S Kjos; D M Nelson; M Desai; M G Ross
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of developmental origins of health and disease     Volume:  4     ISSN:  2040-1752     ISO Abbreviation:  J Dev Orig Health Dis     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2014-07-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101517692     Medline TA:  J Dev Orig Health Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  256-65     Citation Subset:  IM    
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