Document Detail


Reduced Ran expression in Ran(+/-) fibroblasts increases cytokine-stimulated nuclear abundance of the AP-1 subunits c-Fos and c-Jun.
MedLine Citation:
PMID:  20965183     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ran (Ras-related nuclear protein), a Ras family GTPase, is involved in multiple cellular functions, including the regulation of DNA replication, cell cycle progression, nuclear structure formation, RNA processing-exportation, and nuclear protein importation. Ran(+/-) embryonic stem (ES) cells were produced in an attempt to generate Ran null mutant mice. Even after an extremely large number of blastocyst injections, no Ran(+/-) chimeric mice could be generated. Ran(+/-) ES cell-derived fibroblasts showed reduced Ran protein expression, and manifested augmented nuclear abundance of AP-1 factors (c-Jun and c-Fos) upon cytokine stimulation. Our experiments demonstrated that intracellular Ran protein levels controlled the nuclear presence of certain transcription factors, such as c-Fos and c-Jun.
Authors:
Diep Ngoc Thi Pham; Hongyu Luo; Jiangping Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-21
Journal Detail:
Title:  FEBS letters     Volume:  584     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-15     Completed Date:  2010-12-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  4623-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Affiliation:
Laboratory of Immunology, Centre hospitalier de l'Université de Montréal (CRCHUM)-Hôpital Notre-Dame, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Basic-Leucine Zipper Transcription Factors / metabolism*
Cell Nucleus / drug effects*,  metabolism
Cytokines / pharmacology*
Fibroblasts / drug effects*,  metabolism
Gene Expression Regulation / drug effects*
Mice
Protein Subunits / metabolism*
Proto-Oncogene Proteins c-fos / metabolism
Proto-Oncogene Proteins c-jun / metabolism
Transcription Factor AP-1 / metabolism
ran GTP-Binding Protein / metabolism*
Grant Support
ID/Acronym/Agency:
IMH-79565//Canadian Institutes of Health Research; MOP57697//Canadian Institutes of Health Research; MOP69089//Canadian Institutes of Health Research; MOP97829//Canadian Institutes of Health Research; PPP86159//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Basic-Leucine Zipper Transcription Factors; 0/Cytokines; 0/Protein Subunits; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; EC 3.6.5.2/ran GTP-Binding Protein

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