| Reduced oxygenation in human obese adipose tissue is associated with impaired insulin suppression of lipolysis. | |
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MedLine Citation:
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PMID: 20466783 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CONTEXT: Adipose tissue in obese individuals is characterized by reduced capillary density and reduced oxygenation. OBJECTIVE: Our objective was to test whether hypoxia is associated with reduced antilipolytic effect of insulin. PARTICIPANTS, DESIGN, AND SETTING: Twenty-one lean and obese individuals participated in this cross-sectional study at a university-based clinical research center. INTERVENTION: In all subjects, in situ adipose tissue (AT) oxygenation [AT oxygen partial pressure (ATpO2)] was measured with a Clark electrode, insulin sensitivity as well as basal and insulin-suppressed lipolysis (continuous infusion of (2H5)glycerol) were measured during a euglycemic-hyperinsulinemic clamp, and abdominal sc AT biopsies were collected to assess fat cell size (Coulter counting of osmium-fixed cells), capillary density (by staining of histological sections), and gene expression (by quantitative RT-PCR). MAIN OUTCOME MEASURE: In situ ATpO2 was evaluated. RESULTS: The ability of insulin to suppress lipolysis (percent) was positively correlated with insulin sensitivity (r=0.43; P<0.05), ATpO2 (r=0.44; P<0.05), vascular endothelial growth factor mRNA (r=0.73; P<0.01), and capillary density (r=0.75; P<0.01). CONCLUSION: These results indicate that low capillary density and ATpO2 in AT are potentially upstream causes of AT dysfunction. |
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Authors:
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Magdalena Pasarica; Jennifer Rood; Eric Ravussin; Jean-Marc Schwarz; Steven R Smith; Leanne M Redman |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-05-13 |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 95 ISSN: 1945-7197 ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-05 Completed Date: 2010-08-24 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: 4052-5 Citation Subset: AIM; IM |
Affiliation:
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Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, Louisiana 70808, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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ClinicalTrials.gov/NCT00704197 |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorptiometry, Photon Adipose Tissue / blood supply*, metabolism* Analysis of Variance Blood Glucose / metabolism Body Composition Diabetes Mellitus, Type 2 / metabolism Female Glucose Clamp Technique Humans Insulin / metabolism* Insulin Resistance Lipolysis / physiology* Male Obesity / metabolism* Oxygen Consumption Partial Pressure |
| Grant Support | |
ID/Acronym/Agency:
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1P30-DK072476/DK/NIDDK NIH HHS; P20-RR021945/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 11061-68-0/Insulin |
| Comments/Corrections | |
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