Document Detail


Reduced Expression of Psoriasin in Human Airway Cystic Fibrosis Epithelia.
MedLine Citation:
PMID:  22772316     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Psoriasin is a low molecular weight Ca(2+)-binding protein with known antimicrobial activity. Since human airway epithelial cells produce a number of powerful antimicrobial agents as part of their host defence, we investigated whether psoriasin was expressed in human bronchial epithelial cell lines. Expression was investigated in 16HBE14o- cells, derived from a normal individual, and compared to CFBE41o- cells, derived from a cystic fibrosis patient. We also examined psoriasin expression following treatment with factors pertinent to the CF lung- oxidant stress and exposure to pro-inflammatory cytokines. CFBE41o- cells demonstrated much reduced psoriasin levels compared to the 16HBE14o- cells. Increased psoriasin expression was seen following treatment with IL-22 and a cytomix of the pro-inflammatory cytokines IL-1β, TNF-α and IFN-γ; however, the oxidant stressor tert-butyl hydroperoxide had no apparent effect. Over-expression of human psoriasin into both cell lines resulted in increased internalization of Pseudomonas aeruginosa. In conclusion, expression of psoriasin- which has known anti-microbial activity in other systems- appears to be reduced in CFBE410- compared to 16HBE14o- cells, and its expression modified by exposure to pro-inflammatory cytokines.
Authors:
Tiesong Li; Elizabeth A Cowley
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-3
Journal Detail:
Title:  Respiratory physiology & neurobiology     Volume:  -     ISSN:  1878-1519     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101140022     Medline TA:  Respir Physiol Neurobiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Department of Physiology and Biophysics, Dalhousie University, PO Box 15000, Halifax, Nova Scotia, B3H 2R2, CANADA.
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