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Reduced ANXA5 mRNA and protein expression in pregnancies complicated by preeclampsia.
MedLine Citation:
PMID:  24393658     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
INTRODUCTION: The placental anticoagulant protein Annexin A5 (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. The aim of this study was to investigate the expression of ANXA5 in pregnancies complicated by preeclampsia (PE).
MATERIALS AND METHODS: Placental tissue samples were collected from 23 pregnancies with PE and 34 normal pregnancies. ANXA5 mRNA levels were measured by quantitative Real-Time PCR (qPCR), while ANXA5 protein expression was measured by Western Blot (WB) and immunohistochemistry.
RESULTS: ANXA5 mRNA expression in PE samples was lower than 1% of its expression in normal samples (mean±SD: 0.002±0.004 vs. 0.55±0.38, p<0.001), while ANXA5 protein levels in PE samples were approximately at 65% of the average normal expression (mean±SD: 0.53±0.30 vs. 0.81±0.25, p=0.001). Immunohistochemical analysis also verified the above results, since PE placentas tended to have low labelling indexes (LIs), in contrast to controls which demonstrated high LIs (p=0.020). Statistical analysis of the WB data revealed that ANXA5 protein expression was increased in PE smokers vs. PE non-smokers (mean±SD: 0.64±0.23 vs. 0.41±0.33, p=0.027).
CONCLUSIONS: These results suggest that ANXA5 downregulation could be part of the pathophysiology of PE and the possible impairment in coagulation processes, which are seen in pregnancies that demonstrate PE. Further studies may investigate whether ANXA5 could be used as a biomarker for the early detection of PE and for the prediction of its severity.
Authors:
Victor Gourvas; Nikolaos Soulitzis; Anastasia Konstantinidou; Efterpi Dalpa; Ourania Koukoura; Demetrios Koutroulakis; Demetrios A Spandidos; Stavros Sifakis
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-12-22
Journal Detail:
Title:  Thrombosis research     Volume:  -     ISSN:  1879-2472     ISO Abbreviation:  Thromb. Res.     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2014-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Elsevier Ltd. All rights reserved.
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