Document Detail


Reduced AMPK-ACC and mTOR signaling in muscle from older men, and effect of resistance exercise.
MedLine Citation:
PMID:  23000302     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AMP-activated protein kinase (AMPK) is a key energy-sensitive enzyme that controls numerous metabolic and cellular processes. Mammalian target of rapamycin (mTOR) is another energy/nutrient-sensitive kinase that controls protein synthesis and cell growth. In this study we determined whether older versus younger men have alterations in the AMPK and mTOR pathways in skeletal muscle, and examined the effect of a long term resistance type exercise training program on these signaling intermediaries. Older men had decreased AMPKα2 activity and lower phosphorylation of AMPK and its downstream signaling substrate acetyl-CoA carboxylase (ACC). mTOR phosphylation also was reduced in muscle from older men. Exercise training increased AMPKα1 activity in older men, however, AMPKα2 activity, and the phosphorylation of AMPK, ACC and mTOR, were not affected. In conclusion, older men have alterations in the AMPK-ACC and mTOR pathways in muscle. In addition, prolonged resistance type exercise training induces an isoform-selective up regulation of AMPK activity.
Authors:
Mengyao Li; Lex B Verdijk; Kei Sakamoto; Brian Ely; Luc J C van Loon; Nicolas Musi
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-09-19
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  133     ISSN:  1872-6216     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:    2012 Nov-Dec
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-05-23     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  655-64     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ireland Ltd.
Affiliation:
Geriatric Research, Education and Clinical Center, Audie L. Murphy Veterans Hospital, 7400 Merton Minter Blvd, San Antonio, TX 78229, USA.
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MeSH Terms
Descriptor/Qualifier:
AMP-Activated Protein Kinases / metabolism
Acetyl-CoA Carboxylase / metabolism*
Adult
Age Factors
Aged
Aged, 80 and over
Biopsy
Body Weight
Exercise*
Gene Expression Regulation, Enzymologic*
Humans
Male
Middle Aged
Muscles / metabolism*
Phosphorylation
Protein Kinases / metabolism*
Resistance Training*
Signal Transduction
TOR Serine-Threonine Kinases / metabolism*
Grant Support
ID/Acronym/Agency:
K23-AG030979/AG/NIA NIH HHS; R01 DK080157/DK/NIDDK NIH HHS; R01 DK089229/DK/NIDDK NIH HHS; R01-DK089229/DK/NIDDK NIH HHS; R01-DK80157/DK/NIDDK NIH HHS; //Medical Research Council
Chemical
Reg. No./Substance:
EC 2.7.-/Protein Kinases; EC 2.7.1.-/AMP-activated protein kinase kinase; EC 2.7.1.1/MTOR protein, human; EC 2.7.1.1/TOR Serine-Threonine Kinases; EC 2.7.11.1/AMP-Activated Protein Kinases; EC 2.7.11.1/PRKAA1 protein, human; EC 2.7.11.1/PRKAA2 protein, human; EC 6.4.1.2/Acetyl-CoA Carboxylase
Comments/Corrections

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